Early homing of adult mesenchymal stem cells in normal and infarcted isolated beating hearts.

Journal of Cellular and Molecular Medicine
Claudia PennaPasquale Pagliaro

Abstract

Little is known on the early homing features of transplanted mesenchymal stem cells (MSCs). We used the isolated rat heart model to study the homing of MSCs injected in the ventricular wall of a beating heart. In this model all types of cells and matrix elements with their interactions are represented, while external interferences by endothelial/neutrophil interaction and neurohormonal factors are excluded. We studied the morphology and marker expression of MSCs implanted in normal hearts and in the border-zone of infarcted myocardium. Early morphological adaptation of MSC homing differs between normal and infarcted hearts over the first 6 hrs after transplantation. In normal hearts, MSCs migrate very early through the interstitial milieu and begin to show morphological changes. Yet, in infarcted hearts MSCs remain in the site of injection forming clusters of round-shaped cells in the border-zone of the infarcted area. Both in normal and infarcted hearts, immuno-histochemistry and confocal imaging showed that, besides the proliferative marker proliferating cell nuclear agent (PCNA), some transplanted cells early express myoblastic maker GATA-4, and some of them show a VWF immunopositivity. Moreover, a few hours after injection ...Continue Reading

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Citations

May 27, 2010·Journal of Ovarian Research·Svetlana KomarovaLarisa Pereboeva
Mar 19, 2014·Biochimica Et Biophysica Acta·F Di ScipioG N Berta
Oct 26, 2013·Basic Research in Cardiology·Francesca TullioPasquale Pagliaro
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Jan 12, 2013·Journal of Cellular and Molecular Medicine·Claudia PennaPasquale Pagliaro
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Mar 16, 2021·Circulation Journal : Official Journal of the Japanese Circulation Society·Mai WatanabeIchiro Hisatome

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Methods Mentioned

BETA
confocal microscopy
transgenic
fluorescence microscopy

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