Early interactions of Salmonella enterica serovar typhimurium with human small intestinal epithelial explants

Gut
A HaqueG Dougan

Abstract

Salmonella enterica serovar typhimurium (S typhimurium) causes invasive gastroenteritis in humans, a disease involving significant penetration of the intestinal mucosa. However, few studies have been undertaken to investigate this interaction directly using differentiated human gut tissue. To investigate the early interactions of an enteropathogenic strain of S typhimurium with human intestinal mucosa using human intestinal in vitro organ culture (IVOC). Wild-type and mutant derivatives of S typhimurium TML were used to compare interactions with cultured human epithelial cells, bovine ligated loops, and human intestinal IVOC. S typhimurium TML was shown to attach to cultured Caco-2 brush border expressing cells and cause tissue damage and fluid accumulation in a ligated bovine loop model.S typhimurium TML bound predominantly to the mucus layer of human IVOC explants during the first four hours of IVOC incubation. From four to eight hours of IVOC incubation, small but characteristic foci of attaching and invading S typhimurium TML were detected as clusters of bacteria interacting with enterocytes, although there was no evidence for large scale invasion of explant tissues. Ruffling of enterocyte membranes associated with adherent...Continue Reading

References

Nov 1, 1990·The Journal of Infectious Diseases·B B Finlay, S Falkow
Jan 1, 1988·Reviews of Infectious Diseases·R B Chalker, M J Blaser
Aug 1, 1969·The Journal of Clinical Investigation·T H Browning, J S Trier
Jan 1, 1996·Annual Review of Immunology·B D Jones, S Falkow
Jun 9, 2000·Molecular Microbiology·T S Wallis, E E Galyov
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J E Galán
Feb 16, 2002·Immunobiology·M RescignoP Ricciardi-Castagnoli
Jul 16, 2002·Infection and Immunity·R J FitzhenryA D Phillips

❮ Previous
Next ❯

Citations

Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kristin L SchreiberDavid R Brown
Mar 7, 2006·Nature Reviews. Microbiology·Siouxsie WilesBrian Robertson
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Simon KeelyAlan W Baird
Dec 17, 2008·Mucosal Immunology·S K LindenM A McGuckin
Feb 10, 2007·International Journal of Food Microbiology·Shin-Hee Kim, Cheng-I Wei
Jan 24, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Carlos GamazoJ M Irache
Jun 15, 2005·Cellular Microbiology·Alberto Tierrez, Francisco García-del Portillo
Aug 2, 2008·Cellular Microbiology·Roman G GerlachMichael Hensel
May 30, 2006·Microbes and Infection·Kerstin Höner zu BentrupCheryl A Nickerson
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Vanessa Liévin-Le Moal, Alain L Servin
Jun 5, 2019·FEMS Microbiology Reviews·Lucie Etienne-MesminStéphanie Blanquet-Diot
Apr 24, 2020·International Journal of Molecular Medicine·Yong ZhiSangyong Lim
Apr 20, 2018·Frontiers in Cellular and Infection Microbiology·Yuebang Yin, Daoguo Zhou
Sep 11, 2017·ACS Biomaterials Science & Engineering·Sophia M OrbachPadmavathy Rajagopalan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.