Early LC3 lipidation induced by d-limonene does not rely on mTOR inhibition, ERK activation and ROS production and it is associated with reduced clonogenic capacity of SH-SY5Y neuroblastoma cells.

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Laura BerliocchiMaria Tiziana Corasaniti

Abstract

d-Limonene is a natural monoterpene abundant in Citrus essential oils. It is endowed with several biological activities, including inhibition of carcinogenesis and promotion of tumour regression. Recently, d-limonene has been shown to modulate autophagic markers in vitro at concentrations found in vivo, in clinical pharmacokinetic studies. Autophagy is an intracellular catabolic process serving as both an adaptive metabolic response and a quality control mechanism. Because autophagy defects have been linked to a wide range of human pathologies, including neurodegeneration and cancer, there is a need for new pharmacological tools to control deregulated autophagy. To better understand the effects of d-limonene on autophagy, to identify the molecular mechanisms through which this monoterpene rapidly triggers LC3 lipidation and to evaluate the role for autophagy in long-term effects of d-limonene. Human SH-SY5Y neuroblastoma, HepG2 hepatocellular carcinoma and MCF7 breast cancer cells were used. Endogenous LC3-II levels were evaluated by western blotting. Autophagic flux assay was performed using bafilomycin A1 and chloroquine. Intracellular distribution of LC3 protein was studied by confocal microscopy analysis of LC3B-GFP transdu...Continue Reading

Citations

Jun 5, 2019·Neurotoxicity Research·Konrad A SzychowskiJan Gmiński
Sep 11, 2020·International Journal of Environmental Research and Public Health·Michele AntonelliFabio Firenzuoli
Jun 27, 2019·Basic & Clinical Pharmacology & Toxicology·Milad AshrafizadehShima Tavakol
Apr 3, 2020·International Journal of Molecular Sciences·Taejoon KimIm-Soon Lee
Nov 22, 2020·Cell Biology International·Lili ZhaoSurui Sheng
Jan 17, 2021·Antioxidants·MaríaTeresa Donato, Laia Tolosa
Sep 30, 2020·Biochemical and Biophysical Research Communications·Debora GentileMaria Tiziana Corasaniti
Apr 18, 2021·Phytotherapy Research : PTR·Heitor Gomes de Araújo-FilhoJullyana S S Quintans

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