Early-life epileptiform discharges exert both rapid and long-lasting effects on AMPAR subunit composition and distribution in developing neurons

Neuroscience Letters
Qian JiangYuwu Jiang

Abstract

The perinatal period of brain is characterized by dynamic changes in structure and high propensity for epilepsy. Animal models have shown that alterations of AMPA receptor (AMPAR) assembly or function may be related to seizure-induced cell damage, long-lasting impairments in brain development and seizure threshold. However, effects of earlier epileptiform discharges on AMPAR composition and sub-cellular distribution remain understudied. In this study, we analyzed age-dependent variation of relative GluR1 and GluR2 protein levels in primary cultured rat cortical neurons at 7DIV, 12DIV, 17DIV and 21DIV. By inducing a single event of epileptiform activity at 6DIV, we tested the effects of early-life seizure-like insults on AMPAR subunit distribution. We found a significant increase in synaptosomal membrane GluR1 expression in magnesium-free (MGF) medium-treated neurons at each time point detected (p<0.05), while GluR2 expression increased at 7DIV, and declined at 17DIV and 21DIV respectively (p<0.05). That is, a trend of high GluR1 with much lower GluR2 expression on the surface membrane of epileptiform discharges experienced neurons over time in culture was presented. These findings in an in vitro model of early-life seizure may ...Continue Reading

References

Mar 1, 1988·Brain Research·G L Holmes, J L Thompson
Mar 1, 1983·Brain Research·S L MoshéJ Engel
Feb 5, 1999·Progress in Brain Research·K Borges, R Dingledine
Jul 22, 1999·Annals of the New York Academy of Sciences·L K Friedman, A Velísková
Feb 19, 2000·Current Opinion in Neurobiology·S H Lee, M Sheng
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·S Y GroomsR S Zukin
Dec 20, 2000·Brain Research·H TanakaR S Zukin
Apr 3, 2001·Annual Review of Neuroscience·O Steward, E M Schuman
May 2, 2001·Nature Reviews. Neuroscience·R C CarrollR C Malenka
Aug 8, 2001·Trends in Cell Biology·R W WongN Hirokawa
Aug 9, 2001·Journal of Neuroscience Research·J B JensenD S Pickering
Aug 15, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Frances E Jensen
Jul 30, 2003·Methods in Cell Biology·Peter J Meberg, Matthew W Miller
Oct 15, 2003·Neuron·David S Bredt, Roger A Nicoll
Feb 11, 2004·Nature Neuroscience·William JuRobert C Malenka
Nov 20, 2004·Nature Reviews. Neuroscience·Graham L CollingridgeYu Tian Wang
Feb 2, 2006·Neuroscience Letters·Jingmin WangXiru Wu
May 19, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Baosong LiuQi Wan
Nov 18, 2006·Lancet Neurology·Yehezkel Ben-Ari, Gregory L Holmes
Jan 24, 2007·Nature Reviews. Neuroscience·Victor A DerkachThomas R Soderling
Feb 6, 2007·Trends in Neurosciences·Siqiong June Liu, R Suzanne Zukin
Apr 13, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Qian JiangYuwu Jiang

❮ Previous
Next ❯

Citations

Feb 26, 2016·Frontiers in Neuroscience·Helene A FachimMaria L N M Rosa
Apr 2, 2013·Journal of Psychiatric Research·Harald Murck
Nov 26, 2010·Epilepsy Research·Selene LomoioElda Scherini
Jul 6, 2010·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Qian JiangYuwu Jiang
Nov 17, 2009·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Linda K Friedman, Menahem Segal
Dec 30, 2018·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Nadia Kafui Adotevi, Beulah Leitch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.