Early mitochondrial fragmentation is a potential in vitro biomarker of environmental stress

Chemosphere
Daniel PerdizChristian Poüs

Abstract

Mitochondria are essential dynamic organelles that ordinarily balance between fragmentation and fusion. Under stress conditions, a shift toward fragmentation or hyper-fusion is observed as a pro-survival reaction. Fragmentation of mitochondria occurs within minutes or hours after the beginning of the stress and occurs in response to a large number of stress stimuli, including those triggered by environmental contaminants. In this study, we tested whether the change in the mitochondrial phenotype, from tubular to fragmented, could be used as a potential environmental stress biomarker in cells and compared this response with the standard MTT-based viability assay. Firstly, we show that mitochondrial fragmentation induced by selected stressors not only increases with concentrations, but also correlates positively with the cytotoxicity. Secondly, we found that the mitochondrial fragmentation that occurs in the first hour of stress correlated with the viability measured after a 24-h stress, allowing the establishment of a linear relation between mitochondrial fragmentation at 1 h and the predictable associated cytotoxicity of environmental contaminants alone or in mixture. In conclusion, we have succeeded in developing a model of pr...Continue Reading

References

Jul 1, 1997·Critical Reviews in Toxicology·A M Cummings
Aug 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·C Sonnenschein, A M Soto
Feb 19, 2003·Biochemical and Biophysical Research Communications·Naotada IshiharaKatsuyoshi Mihara
Jul 29, 2006·The EMBO Journal·Mark J BarsoumElla Bossy-Wetzel
Mar 16, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lisa D MarroquinYvonne Will
Apr 13, 2007·American Journal of Physiology. Cell Physiology·Werner J H KoopmanPeter H G M Willems
May 4, 2007·Archives of Biochemistry and Biophysics·Insil KimJohn J Lemasters
Oct 12, 2007·Physiological Reviews·Maurice B BurgNatalia I Dmitrieva
Apr 11, 2009·The EMBO Journal·Daniel TonderaJean-Claude Martinou
Jun 17, 2009·Seminars in Cell & Developmental Biology·Giovanni Benard, Mariusz Karbowski
Jul 9, 2009·Physiological Reviews·Marc LiesaAntonio Zorzano
Aug 31, 2010·Free Radical Biology & Medicine·Xiying FanGeorge A Brooks
Sep 22, 2010·Critical Reviews in Toxicology·Marc S BonnefoiJan Willem van der Laan
Nov 26, 2010·Nature Reviews. Molecular Cell Biology·Benedikt Westermann
Sep 1, 2012·Science·Suzanne Hoppins, Jodi Nunnari
Sep 1, 2012·Science·Richard J Youle, Alexander M van der Bliek
Jan 12, 2013·Environmental Science and Pollution Research International·Nicolas CreusotSelim Aït-Aïssa
Jun 5, 2013·Cold Spring Harbor Perspectives in Biology·Alexander M van der BliekSumihiro Kawajiri
Mar 7, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Daniel José BarbosaFélix Carvalho
Mar 13, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bharathi AravamudanY S Prakash
Mar 19, 2014·Environmental Toxicology and Pharmacology·Jaromir Michałowicz
Jan 27, 2015·Cancer & Metabolism·Christopher S Ahn, Christian M Metallo
Jun 25, 2015·Carcinogenesis·Kannan Badri NarayananHyun Ho Park
Jan 18, 2016·Biochemical and Biophysical Research Communications·Naoya HirataYasunari Kanda
Aug 10, 2017·Toxicology·Joel N MeyerAnthony L Luz
Sep 2, 2017·Biochemical and Biophysical Research Communications·Dohyeong KwonSuk-Jo Kang
Feb 21, 2018·Current Biology : CB·Sarah PicklesRichard J Youle
Jun 29, 2018·Nature Cell Biology·Verónica EisnerGyörgy Hajnóczky
Sep 3, 2018·Free Radical Biology & Medicine·Anibal E VercesiEstela N B Busanello

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Citations

Mar 8, 2020·International Journal of Environmental Research and Public Health·Alexis M TemkinLisa M Kamendulis

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