Early onset preeclampsia in a model for human placental trophoblast.

Proceedings of the National Academy of Sciences of the United States of America
Megan A SheridanR Michael Roberts

Abstract

We describe a model for early onset preeclampsia (EOPE) that uses induced pluripotent stem cells (iPSCs) generated from umbilical cords of EOPE and control (CTL) pregnancies. These iPSCs were then converted to placental trophoblast (TB) representative of early pregnancy. Marker gene analysis indicated that both sets of cells differentiated at comparable rates. The cells were tested for parameters disturbed in EOPE, including invasive potential. Under 5% O2, CTL TB and EOPE TB lines did not differ, but, under hyperoxia (20% O2), invasiveness of EOPE TB was reduced. RNA sequencing analysis disclosed no consistent differences in expression of individual genes between EOPE TB and CTL TB under 20% O2, but, a weighted correlation network analysis revealed two gene modules (CTL4 and CTL9) that, in CTL TB, were significantly linked to extent of TB invasion. CTL9, which was positively correlated with 20% O2 (P = 0.02) and negatively correlated with invasion (P = 0.03), was enriched for gene ontology terms relating to cell adhesion and migration, angiogenesis, preeclampsia, and stress. Two EOPE TB modules, EOPE1 and EOPE2, also correlated positively and negatively, respectively, with 20% O2 conditions, but only weakly with invasion; they...Continue Reading

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Citations

Sep 4, 2019·Current Protocols in Stem Cell Biology·Mariko HoriiMana M Parast
Jul 1, 2020·Nature Reviews. Endocrinology·John D AplinMelissa Westwood
Jan 8, 2021·Human Reproduction Update·Samuel OjosnegrosAnna Veiga
Feb 9, 2021·The Journal of Biological Chemistry·Adam MischlerBalaji M Rao
Mar 17, 2021·Scientific Reports·Mariko HoriiMana M Parast
Apr 27, 2021·Biology of Reproduction·J ZhouD J Schust
Jun 15, 2021·Human Reproduction Update·John D Aplin, Carolyn J P Jones
Jul 3, 2021·Frontiers in Cell and Developmental Biology·Adam JaremekStephen J Renaud
Aug 6, 2021·Frontiers in Veterinary Science·Sharon ArcuriTiziana A L Brevini
Aug 28, 2021·Micromachines·Marta CherubiniKristina Haase

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Methods Mentioned

BETA
PCA
ELISA
RNAseq

Software Mentioned

WGCNA
ImageJ
EdgeR

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