Early phase acute myocardial infarct size quantification: validation of the triphenyl tetrazolium chloride tissue enzyme staining technique

American Heart Journal
M C FishbeinW Ganz

Abstract

Gross histochemical delineation of myocardium which has lost dehydrogenase enzyme activity has been shown to facilitate macroscopic recognition of necrotic myocardium. The present study was undertaken to assess the accuracy of the triphenyl tetrazolium chloride (TTC) technique for quantitating myocardial infarct size very early after coronary occlusion. In 16 closed-chest dogs the left anterior descending coronary artery was occluded with an intra-arterial balloon. Twelve dogs were killed 6 hours after occlusion, their hearts excised, cut from apex to base into 1 cm thick slices, and incubated in TTC. Whole-mount histologic sections of each slice were prepared. Myocardial infarct size was measured by planimetry of photographs of each gross slice and histologic section using classical criteria of necrosis. Myocardial infarct size determined in 54 slices by the TTC technique and histologically was similar (25 +/- 16% vs 27 +/- 16% of the left ventricular mass, mean +/- SD) with close correlation between the two methods (r = 0.91). Four dogs were killed 3 hours after occlusion and TTC stained and unstained myocardium was studied by electron microscopy. When the TTC technique identified necrosis so did electron microscopy. Areas id...Continue Reading

References

Jun 1, 1978·Chest·M C FishbeinP R Maroko
Apr 1, 1978·The Journal of Clinical Investigation·S F VatnerP R Maroko

❮ Previous
Next ❯

Citations

Aug 5, 2000·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R S PereiraG Wisenberg
Jan 1, 1984·Acta Neuropathologica·T M LiszczakN T Zervas
Jul 1, 1986·Basic Research in Cardiology·H H KleinH Kreuzer
Jul 1, 1994·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·H WeinsteinJ A Leppo
Jun 2, 2012·Intensive Care Medicine·Nils NeyeWolfgang M Kuebler
Jan 18, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Lalita BabbarPitchai Balakumar
Nov 5, 2004·Basic Research in Cardiology·Rainer SchulzGerd Heusch
Sep 3, 2008·Pflügers Archiv : European journal of physiology·Bo Hjorth BentzenMorten Grunnet
Jul 20, 2012·Molecular and Cellular Biochemistry·Harlokesh Narayan YadavPyare Lal Sharma
Dec 29, 2009·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Qian FuLong Chang
Mar 30, 2012·Journal of Physiology and Biochemistry·Bijun ZhaoDinghua Yi
Mar 1, 1985·The American Journal of Cardiology·P MenascheA Piwnica
Feb 1, 1996·The Annals of Thoracic Surgery·J N NanasS D Moulopoulos
Nov 15, 1988·European Journal of Pharmacology·R ReichhalterW Kobinger
May 30, 1991·European Journal of Pharmacology·P DolaraS Sturlini
Sep 1, 1989·Journal of the American College of Cardiology·R M PeshockJ T Willerson
Apr 1, 1989·Journal of the American College of Cardiology·R W Jeremy, L C Becker
Aug 1, 1991·Journal of the American College of Cardiology·S KobayashiE Corday
Jan 1, 1993·Journal of the American College of Cardiology·F V ModyM Schwaiger
Jul 1, 1993·Journal of the American College of Cardiology·P WhittakerK Przyklenk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.