PMID: 8974040Oct 1, 1996Paper

Early postnatal changes in sarcoplasmic reticulum calcium transport function in spontaneously hypertensive rats

Molecular and Cellular Biochemistry
N FreestoneR Vetter

Abstract

This comparative study investigates the relationship between sarcoplasmic reticulum (SR) calcium(Ca2+)-ATPase transport activity and phospholamban (PLB) phosphorylation in whole cardiac homogenates of spontaneously hypertensive rats (SHR) and their parent, normotensive Wistar Kyoto (WKY) strain during early postnatal development at days 1, 3, 6, 12 and at day 40 to ascertain any difference in SR Ca2+ handling before the onset of hypertension. At day 1, the rate of homogenate oxalate-supported Ca2+ uptake was significantly higher in SHR than in WKY (0.25 +/- 0.02 vs 0.12 +/- 0.01 nmoles Ca2+/mg wet ventricular weight/min, respectively; p < 0.001). This interstrain difference disappeared with further developmental increase in SR Ca2+ transport. Western Blot analysis and a semiquantitative ELISA did not reveal any difference in the amount of immunoreactive PLB (per mg of total tissue protein) between strains at any of the ages studied. In addition, levels of phosphorylated PLB formed in vitro in the presence of radiolabelled ATP and catalytic (C) subunit of protein kinase A did not differ between SHR and WKY at days 1, 3, 6 and 12. At day 40, C subunit-catalyzed formation of 32P-PLB was reduced by 66% (p < 0.001) in SHR when compa...Continue Reading

References

Jan 1, 1977·Annual Review of Physiology·H A Fozzard
Mar 1, 1992·The American Journal of Physiology·M AraiM Periasamy
Dec 1, 1989·Journal of Molecular and Cellular Cardiology·H Tanaka, K Shigenobu
Dec 1, 1988·Experientia·T NakanishiA Takao
Dec 1, 1988·Experientia·M L Kirby
Jan 1, 1982·Annual Review of Physiology·M Tada, A M Katz
Mar 1, 1981·Circulation Research·N C Trippodo, E D Frohlich
Aug 1, 1995·Journal of Molecular and Cellular Cardiology·R VetterH Drexler

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Citations

May 9, 1998·The Journal of Biological Chemistry·S D HartsonR L Matts
Aug 5, 1998·The American Journal of Physiology·J CernohorskýR Vetter
Jun 5, 2001·The Journal of Biological Chemistry·P Pellicena, W T Miller
Sep 25, 2012·Cancer Research·Oliver HantschelGiulio Superti-Furga

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