Early pregnancy dyslipidemia is associated with placental DNA methylation at loci relevant for cardiometabolic diseases.

Epigenomics
Marion OuidirFasil Tekola-Ayele

Abstract

Aim: To identify placental DNA methylation changes that are associated with early pregnancy maternal dyslipidemia. Materials & methods: We analyzed placental genome-wide DNA methylation (n = 262). Genes annotating differentially methylated CpGs were evaluated for gene expression in placenta (n = 64). Results: We found 11 novel significant differentially methylated CpGs associated with high total cholesterol, low-density lipoprotein cholesterol and triglycerides, and low high-density lipoprotein cholesterol. High triglycerides were associated with decreased methylation of cg02785814 (ALX4) and decreased expression of ALX4 in placenta. Genes annotating the differentially methylated CpGs play key roles in lipid metabolism and were enriched in dyslipidemia pathways. Functional annotation found cis-methylation quantitative trait loci for genetic loci in ALX4 and EXT2. Conclusion: Our findings lend novel insights into potential placental epigenetic mechanisms linked with maternal dyslipidemia. Trial Registration: ClinicalTrials.gov, NCT00912132.

Associated Clinical Trials

References

Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S W LawH B Brewer
Sep 15, 1996·Circulation·G C CurhanM J Stampfer
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J KluckenG Schmitz
May 23, 2001·JAMA : the Journal of the American Medical Association·UNKNOWN Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults
Jul 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·B F CravattA H Lichtman
Mar 7, 2003·The Journal of Biological Chemistry·Chi-Liang Eric Yen, Robert V Farese
May 24, 2003·Current Diabetes Reports·D J Pettitt, L Jovanovic
Aug 14, 2003·The Journal of Clinical Endocrinology and Metabolism·Patrick M Catalano
Feb 14, 2006·The Journal of Physiology·Leslie Myatt
Oct 19, 2006·Reproductive Toxicology·Dana C DolinoyRandy L Jirtle
Oct 3, 2007·PLoS Genetics·Jeffrey T Leek, John D Storey
Nov 26, 2008·Chemico-biological Interactions·Bengt PerssonUdo Oppermann
Sep 25, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Anouk van der GraafBarbara A Hutten
Jun 28, 2011·The Journal of Pediatrics·Tanja G M VrijkotteMarcel B Twickler
Nov 30, 2011·Neuro-degenerative Diseases·Dena G Hernandez, Andrew B Singleton
Aug 31, 2012·The Journal of Clinical Endocrinology and Metabolism·Tanja G M VrijkotteMarcel B Twickler
Feb 13, 2013·American Journal of Medical Genetics. Part a·Débora R BertolaMaria Rita Passos-Bueno
May 8, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alexandra C Nica, Emmanouil T Dermitzakis
Oct 8, 2013·Nature Genetics·Cristen J WillerUNKNOWN Global Lipids Genetics Consortium
Oct 12, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Andrée-Anne HoudeLuigi Bouchard
Oct 22, 2013·Genome Biology·Steve Horvath
Jan 31, 2014·Genetic Epidemiology·Richard T BarfieldKaren N Conneely
Apr 12, 2014·Current Pharmaceutical Biotechnology·Emilio Herrera, Henar Ortega-Senovilla
May 13, 2014·Nature Genetics·So-Youn ShinNicole Soranzo
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jan 9, 2015·The Journal of Experimental Biology·Anne TarradeAnne Gabory
Jan 15, 2015·Circulation. Cardiovascular Genetics·Liliane PfeifferMelanie Waldenberger
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Jan 1, 2014·Clinical Lipidology·M Ryan IrvinDonna Arnett

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
RNA-seq

Software Mentioned

R
Illumina
limma
BACON
Functional Mapping and Annotation ( FUMA )
Ingenuity Pathway Analysis ( IPA
Bioconductor
GenomeStudio
R package ‘ prcomp
HaploReg

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.