Early prenatal alcohol exposure alters imprinted gene expression in placenta and embryo in a mouse model

PloS One
Heidi MarjonenNina Kaminen-Ahola

Abstract

Prenatal alcohol exposure (PAE) can harm the embryonic development and cause life-long consequences in offspring's health. To clarify the molecular mechanisms of PAE we have used a mouse model of early alcohol exposure, which is based on maternal ad libitum ingestion of 10% (v/v) ethanol for the first eight days of gestation (GD 0.5-8.5). Owing to the detected postnatal growth-restricted phenotype in the offspring of this mouse model and both prenatal and postnatal growth restriction in alcohol-exposed humans, we focused on imprinted genes Insulin-like growth factor 2 (Igf2), H19, Small Nuclear Ribonucleoprotein Polypeptide N (Snrpn) and Paternally expressed gene 3 (Peg3), which all are known to be involved in embryonic and placental growth and development. We studied the effects of alcohol on DNA methylation level at the Igf2/H19 imprinting control region (ICR), Igf2 differentially methylated region 1, Snrpn ICR and Peg3 ICR in 9.5 embryonic days old (E9.5) embryos and placentas by using MassARRAY EpiTYPER. To determine alcohol-induced alterations globally, we also examined methylation in long interspersed nuclear elements (Line-1) in E9.5 placentas. We did not observe any significant alcohol-induced changes in DNA methylation...Continue Reading

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Citations

Jun 29, 2021·American Journal of Obstetrics and Gynecology·Sarah E SteaneKaren M Moritz
Jul 15, 2021·Experimental and Therapeutic Medicine·Litzy Gisella BermudezAdriana Rojas
Aug 4, 2021·Alcoholism, Clinical and Experimental Research·Sarah E SteaneLisa K Akison
Oct 27, 2021·Alcoholism, Clinical and Experimental Research·Sze Ting Cecilia KwanSandra M Mooney

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Methods Mentioned

BETA
PCR

Software Mentioned

MassARRAY EpiTYPER
SPSS Statistics
EpiDesigner
EpiTYPER

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