Early reaction of astroglial cells in rat hippocampus to streptozotocin-induced diabetes

Neuroscience Letters
Yurii V LebedGalina G Skibo

Abstract

Diabetic patients show impaired brain functions, although underlying mechanisms remain unclear. Little is known as well about early diabetes-related changes in a brain tissue. To investigate them we analyzed the reaction of astroglial cells in the hippocampus of rats rendered diabetic by a single injection of streptozotocin (STZ). Astrocyte count, size and shape as well as levels of glial fibrillary acidic protein (GFAP) and S100b protein were assessed 3, 7 and 14 days after the STZ injection using immunohistochemistry, immuno-enzyme assay and computer-assisted image analysis. The reduced GFAP-positive cell count was found on day 3 when these cells were significantly smaller and less arborized with respect to the control. This tendency reversed on day 7 when more numerous GFAP-positive cells grew in size and became more ramified. S100b-positive cell count changes followed a contrasting pattern, elevating on days 3 and 7 and dropping on day 14. The level of cytoskeletal GFAP changed in parallel with GFAP expression revealed by immunocytochemistry, while cytosolic GFAP level started to increase only 7 days after the STZ injection. At the same time S100b level experienced an elevation on day 3 reaching the peak on day 7 and decrea...Continue Reading

References

Dec 1, 1985·Journal of Developmental and Behavioral Pediatrics : JDBP·C S Holmes, L C Richman
Dec 6, 1997·Endocrinology·D B ZimmerW E Zimmer
Nov 14, 2000·Trends in Neurosciences·W H Gispen, G J Biessels
Mar 20, 2003·Microscopy Research and Technique·Rosario Donato
Apr 20, 2004·European Journal of Pharmacology·Augustina M A BrandsGeert Jan Biessels
Mar 8, 2005·Brain Research·Yanina RevsinAlejandro F De Nicola
Nov 25, 2005·Stroke; a Journal of Cerebral Circulation·Rajesh GargParesh Dandona
Jul 31, 2007·Glia·Luc PellerinPierre J Magistretti
Oct 30, 2007·Nature Neuroscience·Christian S Lobsiger, Don W Cleveland
Mar 12, 2008·Nature Clinical Practice. Endocrinology & Metabolism·Shi Fang YanAnn Marie Schmidt

❮ Previous
Next ❯

Citations

May 15, 2013·Bulletin of Experimental Biology and Medicine·N G LotoshS V Savel'ev
Jan 21, 2012·Rinshō shinkeigaku = Clinical neurology·Shinichi TakahashiNorihiro Suzuki
Dec 3, 2014·Pharmacological Reports : PR·Iwona Chmiel-PerzyńskaEwa M Urbańska
Jul 16, 2010·Cardiovascular Psychiatry and Neurology·Johann SteinerBernhard Bogerts
Dec 20, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Alexander G Nikonenko, Yuriy M Bozhok
Apr 17, 2016·Neurochemical Research·Patrícia NardinCarlos-Alberto Gonçalves
Jun 4, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hui ZhangHao Lei
May 10, 2018·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·P LuoM-J Chang
Sep 6, 2011·Metabolic Brain Disease·Priscylla Nunes de SennaLéder Leal Xavier
May 17, 2014·Metabolic Brain Disease·Aarti NagayachIshan Patro
Feb 21, 2014·F1000Research·Shaimaa N AminIbrahim Mohamady
Jul 25, 2018·Journal of Alzheimer's Disease : JAD·Rui ChenYanjun Zhang
Oct 24, 2021·The European Journal of Neuroscience·Suji LeeSujung Yoon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.