Abstract
The aim of the present investigation was to study the effect of acute swimming training with an anaerobic component on matrix metallopeptidase (MMP) activity and myosin heavy chain gene expression in the rat myocardium. Animals (male Wistar rats, weighing approximately 180 g) were trained for 6 h/day in 3 sessions of 2 h each for 1 to 5 consecutive days (N = 5 rats per group). Rats swam in basins 47 cm in diameter and 60 cm deep filled with water at 33 to 35 degrees C. After the training period a significant increase (P < 0.05) was observed in the heart weight normalized to body weight by about 22 and 35% in the groups that trained for 96 and 120 h, respectively. Blood lactate levels were significantly increased (P < 0.05) in all groups after all training sessions, confirming an anaerobic component. However, lactate levels decreased (P < 0.05) with days of training, suggesting that the animals became adapted to this protocol. Myosin heavy chain-beta gene expression, analyzed by real time PCR and normalized with GAPDH gene expression, showed a significant two-fold increase (P < 0.01) after 5 days of training. Zymography analysis of myocardium extracts indicated a single approximately 60-kDa activity band that was significantly i...Continue Reading
References
Jan 1, 1977·Annual Review of Physiology·J Scheuer, C M Tipton
May 7, 1976·Analytical Biochemistry·M M Bradford
Mar 1, 1990·The Annals of Thoracic Surgery·D RichensC J Hilton
Mar 1, 1986·Journal of Applied Physiology·A M MacIntoshK M Baldwin
Apr 1, 1988·European Heart Journal·B SwynghedauwL Rappaport
Aug 15, 1970·Nature·U K Laemmli
Oct 1, 1982·The Journal of Pediatrics·B S AlpertD L Booker
Sep 8, 1993·Molecular and Cellular Biochemistry·S C TyagiK T Weber
Jan 1, 1994·Journal of Molecular and Cellular Cardiology·P M ButtrickJ Scheuer
Jun 1, 1995·Journal of Molecular and Cellular Cardiology·J P CleutjensK T Weber
Feb 1, 1996·Cardiovascular Research·A M Murphy
Dec 1, 1996·Journal of Applied Physiology·X A BigardA D'Albis
May 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T J Eddinger, D P Meer
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G Goldspink
Dec 22, 1999·Trends in Ecology & Evolution· Benno Meyer-Rochow V
Jan 3, 2001·Journal of Applied Physiology·K M Baldwin, F Haddad
Feb 22, 2001·Journal of Applied Physiology·D Pette
Citations
Sep 7, 2012·BMC Physiology·Luiz A O RochaOctávio L Franco
Nov 8, 2013·Journal of Translational Medicine·Marianna BellafioreAntonio Paoli
Feb 6, 2013·PloS One·Gemma Gay-JordiAnna Serrano-Mollar
Sep 11, 2016·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Dermeval Reis JuniorAndrey Jorge Serra
Jun 27, 2019·The Journal of Sports Medicine and Physical Fitness·Veljko ProkicVladimir Zivkovic