PMID: 3744594Sep 15, 1986Paper

Early spontaneous metastasis in the human epidermoid carcinoma HEp3/chick embryo model: contribution of incidental colonization

International Journal of Cancer. Journal International Du Cancer
J R Gordon, J P Quigley

Abstract

In the experimental model system where human tumor cells (HEp3) are implanted on the chorioallantoic membrane (CAM) of the chick embryo, metastasis of HEp3 cells to the embryonic lung occurs within a few days. Such rapidity in tumor dissemination makes this an attractive and potentially useful model for studying the metastatic process. The model, however, involves microvascular trauma at the site of implantation and thus tumor cells may accidentally enter the circulation during implantation or shortly thereafter. If these cells are the cause of the lung metastasis subsequently measured, the model would be in effect a colonization system and not a true, spontaneous metastasis system. The possible contribution of accidental lung colonization to secondary tumor growth was therefore critically examined in this model. In standard metastasis assays, HEp3 was inoculated onto the CAMs of 10-day embryos, which were then incubated for various periods of time. The embryos' lungs were passaged to a second group of CAMs, incubated for 7 days to allow expansion of any HEp3 cells present, and then assayed for HEp3 cells by both microscopy and measurement of human plasminogen activator (PA) activity. Metastasis was evidenced by PA values above...Continue Reading

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Citations

Sep 10, 2008·Angiogenesis·Anca Maria CimpeanMarius Raica
Sep 1, 1993·The Journal of Cell Biology·P C BrooksJ P Quigley
Sep 14, 2006·Tissue Engineering·Matthias W LaschkeMichael D Menger
Jun 28, 2014·Experimental Cell Research·Domenico Ribatti
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Jan 20, 2009·Cancer Letters·Domenica MangieriDomenico Ribatti
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Oct 20, 2014·Histochemistry and Cell Biology·Maike BuschNicole Dünker
Dec 16, 2004·BioTechniques·Edward Htun van der HorstHeike Allgayer

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