PMID: 6402773Feb 1, 1983Paper

Early sporulation gene spo0F: nucleotide sequence and analysis of gene product

Proceedings of the National Academy of Sciences of the United States of America
H ShimotsuH Saito

Abstract

We have determined the sequence of a 1,162-base-pair DNA fragment containing a spo0F gene which is required for an early stage of sporulation in Bacillus subtilis. The sequence has only one long open reading frame consisting of 173 codons, which has been confirmed to be the spo0F cistron by DNA-mediated transformation and in vitro transcription. In UV-irradiated "maxicells" containing pUBSF13, the plasmid that carries cloned spo0F gene, we have observed the synthesis of a 20-kilodalton polypeptide that is absent from cells carrying a vector plasmid pUB110. The molecular weight of this protein is in agreement with the calculated molecular weight of the spo0F gene product (Mr, 19,065). The putative promoter sequences of spo0F gene were 5' T-A-T-A-A-T 3' at -10 and 5' T-T-G-A-T-T 3' at -35. An octamer sequence, 5' A-A-A-G-G-A-G-G 3', situated 8 base pairs prior to the initiation codon was found to be perfectly complementary with the 3' end of 16S ribosomal RNA. This result offers additional evidence for the proposal by Rabinowitz's group that an extensive mRNA-rRNA interaction is a requirement for efficient translation by B. subtilis ribosomes.

References

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Citations

Jun 1, 1986·Molecular & General Genetics : MGG·T IkeuchiS Tsunasawa
Aug 16, 1984·Biochemical and Biophysical Research Communications·J KudohK Kurahashi
Jan 1, 1990·Biotechnology Advances·Y Murooka
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H Shimotsu, D J Henner
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J BouvierJ Szulmajster
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K A TrachJ A Hoch
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F A FerrariJ A Hoch
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·R H Doi
Nov 25, 1983·Nucleic Acids Research·T Sako, N Tsuchida
Feb 1, 1986·Journal of Biomolecular Structure & Dynamics·V BrendelE N Trifonov
Nov 1, 1986·Journal of Bacteriology·M LewandoskiI Smith
Jun 1, 1985·Microbiological Reviews·P J Piggot, J A Hoch
Feb 1, 1985·Journal of Bacteriology·F A FerrariJ A Hoch

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