Early-stage development of human induced pluripotent stem cell-derived neurons

Journal of Neuroscience Research
Yuki OharaT Shirao

Abstract

Recent advances in human induced pluripotent stem cells (hiPSCs) offer new possibilities for biomedical research and clinical applications. Differentiated neurons from hiPSCs are expected to be useful for developing novel methods of treatment for various neurological diseases. However, the detailed process of functional maturation of hiPSC-derived neurons (hiPS neurons) remains poorly understood. This study analyzes development of hiPS neurons, focusing specifically on early developmental stages through 48 hr after cell seeding; development was compared with that of primary cultured neurons derived from the rat hippocampus. At 5 hr after cell seeding, neurite formation occurs in a similar manner in both neuronal populations. However, very few neurons with axonal polarization were observed in the hiPS neurons even after 48 hr, indicating that hiPS neurons differentiate more slowly than rat neurons. We further investigated the elongation speed of axons and found that hiPS neuronal axons were slower. In addition, we characterized the growth cones. The localization patterns of skeletal proteins F-actin, microtubule, and drebrin were similar to those of rat neurons, and actin depolymerization by cytochalasin D induced similar change...Continue Reading

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Citations

Mar 7, 2017·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Noriko KoganezawaTomoaki Shirao
Feb 6, 2020·Cell Biology International·Narges MahmoodiVafa Rahimi-Movaghar
Dec 4, 2019·Scientific Reports·Carlos BuenoSalvador Martínez
Jan 4, 2020·Journal of Neuroscience Methods·Yuta Ishizuka, Clive R Bramham

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Key Resources (RRID) Mentioned

AB_2532045
AB_430874
SciRes_000136

Software Mentioned

MetaMorph
Meta Imaging
iCell

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