Early stages of conjugation in Escherichia coli.

Journal of Bacteriology
R CurtissD R Stallions

Abstract

We initiated these studies to learn more about the initial events during bacterial conjugation and to optimize conditions for their occurrence. We found that cells in donor cultures grown anaerobically prior to mating have (i) a higher mean number of F pili per cell, (ii) longer F pili, (iii) a higher probability of forming specific pairs with F(-) cells, and (iv) a faster rate of initiation of chromosome transfer than cells grown aerobically. The growth medium for the donor culture also influences these same parameters: a rich medium is superior to a completely synthetic medium. Starvation of donor cells in buffered saline or for a required amino acid results in (i) a loss of F pili, (ii) a loss in the ability of donor-specific phages to adsorb, (iii) a loss of ability to form specific pairs with F(-) cells and to yield recombinants, and (iv) an increase in recipient ability. These changes occur as a function of starvation time, and at rates which are dependent on the conditions of prior growth and starvation of the donor culture. Either treatment provides a rapid method for the production of F(-) phenocopies from donor cultures. Resynthesis of F pili by cells within a starved donor culture commences very soon after restoratio...Continue Reading

References

Jun 23, 1967·Biochemical and Biophysical Research Communications·K A Ippen, R C Valentine
Jan 1, 1969·Annual Review of Microbiology·R Curtiss
Mar 22, 1966·Biochemical and Biophysical Research Communications·R E Krisch, M J Kvetkas
Apr 1, 1966·Journal of Molecular Biology·J D Gross, L G Caro
Jan 1, 1967·Journal of Bacteriology·M Ishibashi
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·L G Caro, M Schnös
Jul 1, 1966·Genetics·M DemerecP E Hartman
Feb 1, 1957·Journal of General Microbiology·K W FISHER
Mar 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·T LOEB, N D ZINDER
Sep 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·C C BRINTONJ CARNAHAN

❮ Previous
Next ❯

Citations

Feb 28, 1977·Molecular & General Genetics : MGG·E A Morgan, S Kaplan
Sep 8, 1975·Molecular & General Genetics : MGG·R A KellnG A O'Donovan
Jan 1, 1972·Molecular & General Genetics : MGG·B M Wilkins, S E Hollom
Apr 17, 1979·Molecular & General Genetics : MGG·J LouarnJ M Louarn
Jul 23, 1976·Molecular & General Genetics : MGG·R A SkurrayP Reeves
Sep 5, 1973·Molecular & General Genetics : MGG·P V Sarathy, O Siddiqi
Nov 8, 2005·Microbiology·Katrin DaehnelPhilip Silverman
Jun 28, 2020·Applied and Environmental Microbiology·Brendan Headd, Scott A Bradford
Jul 22, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D ArutyunovL S Frost
May 11, 2010·Applied and Environmental Microbiology·Jaroslaw E KrólEva M Top
Oct 1, 1973·Acta Pathologica Et Microbiologica Scandinavica. Section B: Microbiology and Immunology·L O FroholmK Bovre
Sep 5, 1973·Molecular & General Genetics : MGG·P V Sarathy, O Siddiqi
Mar 22, 1976·Molecular & General Genetics : MGG·P M Andreoli, H J Nijkamp
May 18, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Philip M Silverman, Margaret B Clarke
May 9, 1970·Nature·J H Lancaster, J J Skvarla
Jan 1, 1975·Progress in Biophysics and Molecular Biology·M TomoedaT Date
Oct 22, 1975·Molecular & General Genetics : MGG·T C Lo, B D Sanwal
Apr 1, 1998·Evolution; International Journal of Organic Evolution·Paul E TurnerRichard E Lenski
Apr 6, 2017·Frontiers in Microbiology·Akshay MalwadeBrian P Ingalls
Sep 1, 1971·Journal of Bacteriology·C P Novotny, K Lavin
Jan 1, 1973·Journal of Bacteriology·R A Skurray, P Reeves
Jan 1, 1973·Journal of Bacteriology·E Meynell, A Ewins
Dec 1, 1972·Bacteriological Reviews·K B Low
Jan 1, 1974·Journal of Bacteriology·R A Skurray, P Reeves
Jul 1, 1972·Journal of Bacteriology·J T Ou, T F Anderson
Mar 1, 1974·Journal of Bacteriology·R E Bryant, P S Sypherd
Aug 1, 1973·Journal of Bacteriology·R J Kadner, G L Liggins
Jun 1, 1970·Journal of Bacteriology·J T Ou, T F Anderson
Feb 1, 1975·Journal of Bacteriology·E F TresguerresR H Pritchard
Feb 1, 1975·Journal of Bacteriology·S Chakrabarti, L Gorini
Mar 1, 1975·Journal of Bacteriology·S Hiraga, T Saitoh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.