Early stem cell engraftment predicts late cardiac functional recovery: preclinical insights from molecular imaging.

Circulation. Cardiovascular Imaging
Junwei LiuJoseph C Wu

Abstract

Human cardiac progenitor cells have demonstrated great potential for myocardial repair in small and large animals, but robust methods for longitudinal assessment of their engraftment in humans is not yet readily available. In this study, we sought to optimize and evaluate the use of positron emission tomography (PET) reporter gene imaging for monitoring human cardiac progenitor cell (hCPC) transplantation in a mouse model of myocardial infarction. hCPCs were isolated and expanded from human myocardial samples and stably transduced with herpes simplex virus thymidine kinase (TK) PET reporter gene. Thymidine kinase-expressing hCPCs were characterized in vitro and transplanted into murine myocardial infarction models (n=57). Cardiac echocardiographic, magnetic resonance imaging and pressure-volume loop analyses revealed improvement in left ventricular contractile function 2 weeks after transplant (hCPC versus phosphate-buffered saline, P<0.03). Noninvasive PET imaging was used to track hCPC fate over a 4-week time period, demonstrating a substantial decline in surviving cells. Importantly, early cell engraftment as assessed by PET was found to predict subsequent functional improvement, implying a "dose-effect" relationship. We iso...Continue Reading

References

Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·M E BlackL A Loeb
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·S S GambhirH R Herschman
Mar 22, 2002·The New England Journal of Medicine·Arthur J MossUNKNOWN Multicenter Automatic Defibrillator Implantation Trial II Investigators
May 7, 2003·Circulation·Dara L KraitchmanJeff W M Bulte
Aug 13, 2003·Circulation·Jonathan M HillRobert J Lederman
May 1, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Vladimir PonomarevJuri Gelovani
May 30, 2007·Archives of Internal Medicine·Ahmed Abdel-LatifBuddhadeb Dawn
Aug 22, 2007·Proceedings of the National Academy of Sciences of the United States of America·Claudia BearziPiero Anversa
Jun 21, 2008·Netherlands Heart Journal : Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·P van VlietM-J Goumans
Sep 17, 2008·Circulation·Volker SchächingerAndreas M Zeiher
Oct 10, 2008·Circulation·Koen E A van der BogtJoseph C Wu
Nov 11, 2008·Journal of the American College of Cardiology·Joseph C Wu
Nov 19, 2008·Nature Clinical Practice. Oncology·Shahriar S YaghoubiSanjiv S Gambhir
Feb 19, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Amer M NajjarJuri G Gelovani
Apr 4, 2009·Journal of the American College of Cardiology·Zongjin LiJoseph C Wu
Mar 10, 2010·Journal of Molecular and Cellular Cardiology·Darryl R DavisEduardo Marbán
Jul 21, 2010·Journal of Molecular and Cellular Cardiology·Sveva BolliniPaul R Riley
Feb 11, 2011·Journal of Cellular and Molecular Medicine·Angelique A M van OorschotMarie-José Goumans
Oct 1, 2011·Circulation Research·Patricia K NguyenJoseph C Wu

❮ Previous
Next ❯

Citations

Jun 22, 2013·Transplantation Research·Diana F Anthony, Paul G Shiels
Mar 7, 2014·Antioxidants & Redox Signaling·Polina GoichbergAnnarosa Leri
Apr 9, 2013·Trends in Cardiovascular Medicine·Ian Y Chen, Joseph C Wu
Feb 18, 2016·Stem Cells International·Mayra Lorena MoreiraPaulo Henrique Rosado-de-Castro
Feb 18, 2016·Stem Cells International·Maximiliano I SchaunMelissa M Markoski
Nov 23, 2015·Cardiology Clinics·James T Thackeray, Frank M Bengel
Aug 2, 2014·Journal of the American College of Cardiology·Veronica Sanchez-FreireJoseph C Wu
Sep 7, 2016·Diagnostics·Jonathan A Pan, Michael Salerno
Nov 1, 2016·Stem Cells International·Paula MüllerRobert David
Dec 13, 2017·Circulation·Diamantis I TsilimigrasKonstantinos S Mylonas
Feb 17, 2018·Circulation. Cardiovascular Imaging·Jonathan R Lindner, Jeanne Link
Apr 7, 2018·The Egyptian Heart Journal : (EHJ) : Official Bulletin of the Egyptian Society of Cardiology·Maha H El SebaieA M Fadel
May 25, 2013·The Journal of Biological Chemistry·Peter VandersliceRichard A F Dixon
May 22, 2020·Journal of Translational Internal Medicine·Bin Duan
Jul 31, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Qiujun YuFeng Cao
May 10, 2017·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Amnon Bar-ShirAssaf A Gilad
Jun 30, 2019·Oxidative Medicine and Cellular Longevity·Yinuo GuanBin Liu
Sep 22, 2020·Nuclear Medicine and Biology·Aditya BansalTimothy R DeGrado
Aug 4, 2021·Journal of Cardiovascular Translational Research·Olivier SchusslerYves Lecarpentier

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.