PMID: 2863322Sep 1, 1985

Early T lymphocytes. Differentiation in vivo of adult intrathymic precursor cells

The Journal of Experimental Medicine
B J FowlkesT M Chused

Abstract

A minor subpopulation of adult murine thymocytes (less than 5%) that is Lyt-2-, L3T4-, and expresses low levels of Ly-1 (designated dLy-1 [dull] thymocytes) has been identified, isolated, and characterized. This study assesses the differentiation potential of dLy-1 thymocytes in the thymus in vivo. Using multiparameter flow cytometry, radiation chimeras of C57BL/6 mice congenic at the Ly-1 or Ly-5 locus, and allelic markers to discriminate host and donor, we showed that transferred dLy-1 cells were able to generate thymocytes expressing both cortical and medullary phenotypes in a sequential manner. The proportion of donor-derived thymocytes obtained was directly related to the number of dLy-1 thymocytes transferred. Transfer of purified Lyt-2+ or Lyt-2+ + L3T4+ thymocytes, which constitute greater than 94% of total thymocytes, failed to generate any donor-derived thymocytes in irradiated recipients. Transfer of bone marrow (BM) cells produced the same sequential pattern of differentiation as that produced by dLy-1 cells, but was delayed by 4-5 d. Transferred dLy-1 thymocytes exhibited a limited capacity for self-renewal, and resulted in a single wave of differentiation in irradiated hosts. Thus, thymic repopulation by donor-der...Continue Reading

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Related Concepts

T-Lymphocyte
Colony-Forming Units, Hematopoietic
Mice, Inbred C3H
Cell Differentiation Process
Ptprc
Cd5
sens
Hemopoietic Stem Cell Transplant
Antigens, Ly
Bone Marrow Cell Transplantation

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