Nov 26, 2008

Early tissue patterning recreated by mouse embryonic fibroblasts in a three-dimensional environment

Tissue Engineering. Part a
Lluís QuintanaCarlos E Semino

Abstract

Cellular self-organization studies have been mainly focused on models such as Volvox, the slime mold Dictyostelium discoideum, and animal (metazoan) embryos. Moreover, animal tissues undergoing regeneration also exhibit properties of embryonic systems such as the self-organization process that rebuilds tissue complexity and function. We speculated that the recreation in vitro of the biological, biophysical, and biomechanical conditions similar to those of a regenerative milieu could elicit the intrinsic capacity of differentiated cells to proceed to the development of a tissue-like structure. Here we show that, when primary mouse embryonic fibroblasts are cultured in a soft nanofiber scaffold, they establish a cellular network that causes an organized cell contraction,proliferation, and migration that ends in the formation of a symmetrically bilateral structure with a distinct central axis. A subset of mesodermal genes (brachyury, Sox9, Runx2) is upregulated during this morphogenetic process. The expression of brachyury was localized first at the central axis, extending then to both sides of the structure. The spontaneous formation of cartilage-like tissue mainly at the paraxial zone followed expression ofSox9 and Runx2. Becaus...Continue Reading

  • References41
  • Citations29

Mentioned in this Paper

Embryo
Regenerative Medicine
SOX9 gene
Specimen Type - Fibroblasts
Physarum polycephalum
Cell Motility
Core Binding Factor Alpha 1 Subunit
RUNX2 gene
Animal Tissue, NOS
Cell Culture Techniques

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