Early transcriptional response of human ovarian and fallopian tube surface epithelial cells to norepinephrine

Scientific Reports
Anxhela GjyshiAlvaro N A Monteiro

Abstract

Evidence from human and animal studies suggests that chronic behavioral stress and resulting activation of the sympathetic nervous system may influence initiation and progression of tumors. However, the underlying mechanisms for these observations are poorly understood. The purpose of this study is to explore the effects of adrenergic signaling on cell line models derived from normal cells presumed to originate epithelial ovarian cancers. Here we explored the effects of the stress-related hormone, norepinephrine, on the transcriptional program of normal immortalized ovarian (iOSE) and fallopian tube (iFTSEC) surface epithelial cells. Analysis of RNA-Seq data of treated and untreated cells revealed a significant overlap between the responses in iOSE and iFTSEC cells. Most genes modulated by norepinephrine in ovarian and fallopian tube epithelial cells are already expressed in normal ovarian and fallopian tissue and cells. For several genes, expression changes were reflected at the protein level. Genes in immune-related and developmental pathways were enriched in the set of genes modulated by norepinephrine. We identified HOXA5, SPIB, REL, SRF, SP1, NFKB1, MEF2A, E2F1, and EGR1 transcription factor binding sites to be highly enri...Continue Reading

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Methods Mentioned

BETA
RNA-Seq
FAIRE-Seq
immunoprecipitation
environmental stress
PCR
electrophoresis
Assay

Software Mentioned

HTSeq
PANTHER
Metacore
GEO Profiles
Bioconductor
oPOSSUM
GeneGo MetaCore
NIH ImageJ ( Imaging processing and analysis Java
R
DESeq2

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