Early upregulation of cyclooxygenase-2 in human papillomavirus type 16 and telomerase-induced immortalization of human esophageal epithelial cells

Journal of Gastroenterology and Hepatology
Ze-Hao ZhuangBenjamin C-Y Wong

Abstract

Cyclooxygenase-2 (COX-2) plays an important role in the carcinogenesis of esophageal squamous cell carcinoma (ESCC). However, it is not clear whether COX-2 is involved in the early or late stage of the development of ESCC. The aim of this study was to investigate the role of COX-2 in the carcinogenesis of ESCC by an immortalized esophageal epithelial cell line. Human papillomavirus type 16 (HPV16)-E6/E7 and human telomerase reverse transcriptase (hTERT) transfection were used for immortalization of esophageal epithelial cells. COX-2-specific RNA interference was used for the inhibition of COX-2 expression. An immortalized esophageal epithelial cell line, NE6-E6E7/hTERT, was established, which had high proliferation activity but failed to induce colony formation in soft agar. COX-2 expression was upregulated in the early process of immortalization, while COX-2 small interfering RNA (siRNA) decreased the Bcl-2 expression, increased the expression of Bax, and induced cell-cycle arrest at the G0/G1 phase in NE6-E6E7/hTERT cells. Expressions of p53, cyclinD1, and the ratio of hyperphosphorylated-RB/hypophosphorylated-RB were progressively increased after E6E7 and the subsequent hTERT transfections. These changes were accompanied by ...Continue Reading

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Citations

Dec 29, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Maria Teresa Rizzo
Aug 7, 2010·Biochemical and Biophysical Research Communications·Sawali R SudarshanXuefeng Liu
May 17, 2014·Pathology Oncology Research : POR·Mohammed Amjed AlsaeghSheng Rong Zhu
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Oct 26, 2016·Molecular Medicine Reports·Fa-Min ZengEn-Min Li
Jul 24, 2018·Gastroenterology Research and Practice·Sung Hoo KimKang Hong Lee

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