Easy reuse of magnetic cross-linked enzyme aggregates of lipase B from Candida antarctica to obtain biodiesel from Chlorella vulgaris lipids

Journal of Bioscience and Bioengineering
Enrique A PicóMaría J Llama

Abstract

In this work, magnetic cross-linked enzyme aggregates (mCLEAs) of CALB (lipase B from Candida antarctica) were prepared and characterized. Moreover, a method for an easy, sustainable and economic extraction of lipids from nitrogen-starved cells of Chlorella vulgaris var L3 was developed. Then, the extracted lipids (oils and free fatty acids, FFAs) were converted to biodiesel using mCLEAs and chemical acid catalysis. Among several lipid extraction methods, saponification was selected given the amount of wet microalgal biomass it can process per unit of time, its low market value, and because it allows for the use of less toxic solvents. A biodiesel conversion of 80.2 ± 4.4% was obtained by chemical catalysis (1 h at 100°C) using FFAs and methanol as the alkyl donor. However, a biodiesel conversion of more than 90% (3 h at 30°C) was obtained using mCLEAs and methanol. Both chemical and enzymatic catalysts gave biodiesel with similar fatty acid alkyl ester (FAAE) composition. Methanol, at 15% (v/v) or higher concentration, caused a decrease of lipase activity and a concomitant increase in the size of mCLEA aggregates (up to 2 μm), as measured by dynamic light scattering (DLS). The magnetic character of the novel biocatalyst permit...Continue Reading

Citations

Oct 10, 2019·Biotechnology and Applied Biochemistry·João H C WancuraJosé Vladimir de Oliveira
Dec 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Margarita Díaz RamosPaulo Waldir Tardioli
Aug 4, 2019·Applied Microbiology and Biotechnology·Danielle Gonçalves FilhoCarla Zanella Guidini
Nov 5, 2020·International Journal of Biological Macromolecules·Muhammad Bilal, Hafiz M N Iqbal

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