Easy strategy used to detect the genetic variability in chickpea (Cicer arietinum L.)

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
E Valadez-Moctezuma, A J Cabrera-Hidalgo

Abstract

A priority in the management and use of elite plant materials for breeding has been based on molecular markers or DNA sequencing of entire genomes, in order to perform genetic differentiation which is still quite costly. Chickpea (Cicer arietinum) is one of the species with genomic monotony and very low polymorphism, and its detection even with DNA markers has not been easy. In germplasm banks, the genetic distinction is a priority in order to use properly selected lines. In this study, 57 chickpea accessions from a germplasm bank were analyzed by using nrRAMP (non-radioactive Random Amplified Microsatellite Polymorphism) markers, and their genetic variability was determined. Our results showed DNA polymorphisms, which are enough to differentiate between the accessions and between C. arietinum and Cicer reticulatum (out-group); this last wild species is closely related to chickpea. We concluded that the nrRAMP technique was an effective and a highly useful method to assess the genetic diversity and variability among closely related plants, such as chickpea; in addition, this technique can be easily implemented in laboratories.

References

Aug 11, 1994·Nucleic Acids Research·K S WuP A Scolnik
Feb 1, 1996·Genome Génome / Conseil National De Recherches Canada·M P Sánchez de la HozE Ferrer
Dec 28, 2006·Cell·John F DoebleyBruce D Smith
Jul 29, 2010·Genetics and Molecular Research : GMR·F AhmadS Bahadur
Mar 5, 2013·Annual Review of Plant Biology·Kenneth M Olsen, Jonathan F Wendel
Jul 1, 1995·World Journal of Microbiology & Biotechnology·P Winter, G Kahl

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Methods Mentioned

BETA
PCR
electrophoresis
ISSR

Software Mentioned

UPGMA
NTSYSpc
nrRAMP

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