Easy to use and reliable technique for online dissolved oxygen tension measurement in shake flasks using infrared fluorescent oxygen-sensitive nanoparticles

Microbial Cell Factories
David FlitschJochen Büchs

Abstract

Despite the progressive miniaturization of bioreactors for screening purposes, shake flasks are still widespread in biotechnological laboratories and industry as cultivation vessels. Shake flasks are often applied as the first or second step in applications such as strain screening or media optimization. Thus, there are ongoing efforts to develop online measurement techniques for shake flasks, to gain as much information as possible about the cultured microbial system. Since dissolved oxygen tension (DOT) is a key experimental parameter, its accurate determination during the course of experiment is critical. Some of the available DOT measurement techniques can lead to erroneous measurements or are very difficult to handle. A reliable and easy to use DOT measurement system, based on suspended oxygen-sensitive nanoparticles, is presented in this work. In a cultivation of Kluyveromyces lactis, a new DOT measurement technique via suspended oxygen-sensitive nanoparticles was compared with the conventional DOT measurement via fixed sensor spots. These experiments revealed the main disadvantage of applying sensor spots. With further cultivations of Escherichia coli and Hansenula polymorpha, the new measurement technique was successful...Continue Reading

References

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Citations

Mar 28, 2018·Applied Microbiology and Biotechnology·Masato Takahashi, Hideki Aoyagi
Jun 1, 2018·Applied Microbiology and Biotechnology·Masato Takahashi, Hideki Aoyagi
Nov 1, 2016·Journal of Biological Engineering·David FlitschJochen Büchs
Jun 3, 2021·Plants·Xiaoxiao Shi, Arnold Bloom

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