Easy to use plastic optical fiber-based biosensor for detection of butanal

PloS One
Nunzio CennamoSabato D'Auria

Abstract

The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach. To this end, a porcine odorant-binding protein was used in connection with surface plasmon resonance transduction in a plastic optical fiber tool for the selective detection of butanal by a competitive assay. This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform. The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.

References

Jan 5, 2007·Analytical and Bioanalytical Chemistry·K Scott Phillips, Quan Cheng
Sep 12, 2007·Biochemistry·Maria StaianoKonstantin K Turoverov
Oct 19, 2010·Biosensors & Bioelectronics·Abdennour AbbasQuan Cheng

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Citations

Apr 20, 2017·Sensors·Dario CompagnoneAntonella Tajani
Sep 6, 2020·Applied Microbiology and Biotechnology·Nathália F BritoAna Claudia A Melo
Feb 6, 2021·Critical Reviews in Biotechnology·Filipa GonçalvesArtur Cavaco-Paulo

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Methods Mentioned

BETA
biosensor
Surface Plasmon Resonance
chip
AFM
chips
competition
atomic force microscopy

Software Mentioned

Matlab
Ocean Optics

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