EAT/mcl-1 expression in the human embryonal carcinoma cells undergoing differentiation or apoptosis

Experimental Cell Research
M SanoJ Hata

Abstract

Differentiation and apoptosis are precisely regulated events in early embryogenesis. Retinoic acid-induced differentiation in the embryonal carcinoma (EC) cell line NCR-G3 triggers concurrent induction of apoptosis. Using this system, which serves as a model of early embryogenesis, the expression of various bcl-2-related genes was analyzed as these genes display either positive or negative regulatory effects on apoptosis. EAT/mcl-1, an antiapoptotic bcl-2-related gene and immediate early gene, was dramatically expressed at an early stage of NCR-G3 differentiation. Bcl-xL, another antiapoptotic gene, was induced at a middle stage of differentiation and then gradually decreased to basal level. Expression of Bax, a proapoptotic molecule, was detected at a high level and remained relatively constant. Meanwhile, Bcl-2 and Bcl-xS were below detectable levels throughout the various stages of differentiation. As the balance of bcl-2 genes is a crucial regulatory step in apoptosis, the results suggest that EAT and Bax likely regulate apoptosis in the early stages of differentiation. In later stages of differentiation, down-regulation of EAT was found to coincide with a gradual increase in apoptosis of NCR-G3 cells. Furthermore, use of t...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B Mintz, K Illmensee
Dec 1, 1992·The Journal of Cell Biology·H M McBrideG C Shore
Jun 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H ShimizuR A Eady
Dec 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P MonaghanM F Greaves
Aug 15, 1980·Science·G R Martin
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·T W SedlakS J Korsmeyer
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M KozopasR W Craig
Apr 20, 1995·Nature·T ChittendenB C Guild
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·T SatoH G Wang
Jan 1, 1994·The Journal of Cell Biology·J C Reed
Apr 1, 1996·The Journal of Experimental Medicine·N ZamzamiG Kroemer
Feb 1, 1996·Cell Structure and Function·T YamadaJ Hata
Oct 1, 1996·The Journal of Experimental Medicine·S A SusinG Kroemer
Nov 15, 1996·Genes & Development·K WangS J Korsmeyer
Jan 7, 1998·The Journal of Cell Biology·K G WolterR J Youle
May 23, 1998·Annual Review of Immunology·D T Chao, S J Korsmeyer
Sep 12, 1998·Science·J M Adams, S Cory
Oct 2, 1998·Advances in Immunology·A J MinnC B Thompson
Oct 15, 1998·Molecular Reproduction and Development·A JurisicovaS L Varmuza
Apr 15, 2000·The Journal of Biological Chemistry·C D BingleM K Whyte

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Citations

Jul 8, 2009·Journal of Cellular Physiology·Hidekazu TakahashiAkihiro Umezawa
Jan 22, 2009·Molecular Nutrition & Food Research·Jirina HofmanováAlois Kozubík
Jun 5, 2003·Molecular and Cellular Endocrinology·Kenichi MatsushitaAkihiro Umezawa

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