Ebf2 is a selective marker of brown and beige adipogenic precursor cells

Proceedings of the National Academy of Sciences of the United States of America
Wenshan WangPatrick Seale


Brown adipocytes and muscle and dorsal dermis descend from precursor cells in the dermomyotome, but the factors that regulate commitment to the brown adipose lineage are unknown. Here, we prospectively isolated and determined the molecular profile of embryonic brown preadipose cells. Brown adipogenic precursor activity in embryos was confined to platelet-derived growth factor α(+), myogenic factor 5(Cre)-lineage-marked cells. RNA-sequence analysis identified early B-cell factor 2 (Ebf2) as one of the most selectively expressed genes in this cell fraction. Importantly, Ebf2-expressing cells purified from Ebf2(GFP) embryos or brown fat tissue did not express myoblast or dermal cell markers and uniformly differentiated into brown adipocytes. Interestingly, Ebf2-expressing cells from white fat tissue in adult animals differentiated into brown-like (or beige) adipocytes. Loss of Ebf2 in brown preadipose cells reduced the expression levels of brown preadipose-signature genes, whereas ectopic Ebf2 expression in myoblasts activated brown preadipose-specific genes. Altogether, these results indicate that Ebf2 specifically marks and regulates the molecular profile of brown preadipose cells.


Apr 17, 2001·Experimental Cell Research·W E Blanco-BoseH M Blau
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Oct 25, 2006·Molecular and Cellular Biology·Maria A JimenezEvan D Rosen
Sep 18, 2007·Genesis : the Journal of Genetics and Development·Mandar Deepak MuzumdarLiqun Luo
Aug 23, 2008·Nature·Patrick SealeBruce M Spiegelman
Sep 20, 2008·Science·Wei TangJonathan M Graff
Oct 7, 2008·Cell·Matthew S RodehefferJeffrey M Friedman
Mar 5, 2010·Nature·Rana K GuptaBruce M Spiegelman
Jul 20, 2010·Genesis : the Journal of Genetics and Development·Christoph Lepper, Chen-Ming Fan
Dec 3, 2010·The Journal of Clinical Investigation·Patrick SealeBruce M Spiegelman
Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tim J SchulzYu-Hua Tseng
Nov 21, 2012·PloS One·Louis Z SharpShingo Kajimura
Feb 26, 2013·Nature Cell Biology·Ryan Berry, Matthew S Rodeheffer
Mar 19, 2013·Cell Metabolism·Sona RajakumariPatrick Seale
Apr 23, 2013·Nature Medicine·Martin E LidellSven Enerbäck
Aug 31, 2013·Developmental Cell·Jun Hong ParkSean Bong Lee
Oct 9, 2013·Nature Medicine·Matthew Harms, Patrick Seale
Dec 3, 2013·Cell Reports·Irina G ShabalinaJan Nedergaard


Feb 15, 2016·Trends in Cell Biology·Joan Sanchez-GurmachesDavid A Guertin
Jan 15, 2016·Cytokine & Growth Factor Reviews·Lovorka GrgurevicSlobodan Vukicevic
Sep 4, 2015·Molecular Nutrition & Food Research·Vasso ApostolopoulosBarbora de Courten
Sep 28, 2014·Proceedings of the National Academy of Sciences of the United States of America·Matthew D Lynes, Yu-Hua Tseng
Oct 8, 2015·Cell Metabolism·Shingo KajimuraPatrick Seale
Sep 1, 2015·Frontiers in Endocrinology·Jun Hong ParkSean Bong Lee
Oct 23, 2015·Genes & Diseases·Matthew D Lynes, Yu-Hua Tseng
Jul 21, 2015·Cell Reports·Jianbo NaRoss L Cagan
May 26, 2015·Frontiers in Endocrinology·Jon Dempersmier, Hei Sook Sul
Mar 26, 2015·The Journal of Endocrinology·Margo P EmontJun Wu
Apr 9, 2015·Trends in Genetics : TIG·Jun WuJoseph R McDermott
Mar 26, 2015·Trends in Endocrinology and Metabolism : TEM·Pengpeng Bi, Shihuan Kuang
Feb 11, 2015·Biochimica Et Biophysica Acta·Catriona HiltonKatherine E Pinnick
Apr 14, 2016·Genesis : the Journal of Genetics and Development·Brian G Condie
Jan 6, 2016·Journal of Cellular Physiology·Petar D PetrovJoan Ribot
Jun 3, 2016·Nature Reviews. Molecular Cell Biology·Takeshi InagakiShingo Kajimura
Jun 21, 2016·Radiology·Srihari C SampathMartin Torriani
Aug 25, 2016·Nature Reviews. Molecular Cell Biology·Wenshan Wang, Patrick Seale
Aug 30, 2016·Cell Metabolism·Svetlana Altshuler-KeylinShingo Kajimura
Oct 6, 2016·Pflügers Archiv : European journal of physiology·Yong ChenAlexander Pfeifer
Apr 5, 2016·Acta Physiologica·A RodríguezG Frühbeck
Feb 17, 2017·Genes & Development·Chelsea HeplerRana K Gupta
Aug 16, 2017·Nature Cell Biology·Yuta HiraikeTakashi Kadowaki
Aug 31, 2018·International Journal of Molecular Sciences·María Del Carmen GarcíaCarlos Diéguez
Dec 21, 2018·Obesity·Suzanne N Shapira, Patrick Seale
Jan 5, 2019·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Ana M Blázquez-MedelaKristina I Boström
Nov 6, 2018·Nature Reviews. Molecular Cell Biology·Matthew J Emmett, Mitchell A Lazar
Nov 13, 2014·Molecular Endocrinology·Stefano BartesaghiXiao-Rong Peng
Nov 17, 2016·Endocrine Reviews·Susan M van den BergEsther Lutgens
Jul 12, 2019·Journal of Diabetes Investigation·Allah Nawaz, Kazuyuki Tobe
Oct 14, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Suriyan PonnusamyRamesh Narayanan
Apr 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Soumya Jaya DivakaranSona Rajakumari
May 27, 2020·Bioscience Reports·Sunhye ShinYuwei Jiang
Jun 11, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yangyang HuangJian Shen
Mar 1, 2019·Frontiers in Physiology·Martín AlcaláMarta Viana
Apr 3, 2019·Molecular and Cellular Biology·Ji-Eun LeeKai Ge
Mar 3, 2020·Frontiers in Cell and Developmental Biology·Xiaoting SunYing Ye
Jun 17, 2020·The Biochemical Journal·Alice E Pollard, David Carling
Aug 2, 2017·Annals of the New York Academy of Sciences·Matthew D Lynes, Yu-Hua Tseng
Apr 6, 2019·Development·Zachary L Sebo, Matthew S Rodeheffer
Jan 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Suriyan PonnusamyRamesh Narayanan
Sep 30, 2020·Journal of Cellular Physiology·Liyi Wang, Tizhong Shan
Oct 6, 2020·Frontiers in Endocrinology·Atefeh Rabiee
Jan 1, 2015·Temperature : Multidisciplinary Biomedical Journal·Jeff Ishibashi, Patrick Seale
Dec 10, 2016·The Journal of Endocrinology·Liping Luo, Meilian Liu
Feb 25, 2017·Stem Cells·Deanna P PorrasAnthony Scimè
Apr 28, 2017·Genes & Development·Jiexin Wang, Peter Tontonoz
Apr 18, 2017·Nature Immunology·Kyoung-Jin ChungTriantafyllos Chavakis
Jan 6, 2019·Nature Reviews. Molecular Cell Biology·Alexandra L Ghaben, Philipp E Scherer
Feb 23, 2018·Frontiers in Physiology·Staffan HildebrandAlexander Pfeifer
Sep 22, 2019·Diabetes·Mengle ShaoRana K Gupta
Jun 23, 2020·Journal of Molecular Cell Biology·Hai-Bin Ruan
Jan 4, 2021·Cell Biochemistry and Function·Mariane Izabella Abreu de MeloDawidson Assis Gomes
Feb 2, 2021·Medicinal Research Reviews·Milena DeptułaMichał Pikuła
Nov 25, 2020·Metabolites·Fernando Lizcano, Felipe Arroyave
Mar 14, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Sona Rajakumari, Simran Srivastava
Mar 18, 2021·Frontiers in Cell and Developmental Biology·Miriam A HolzmanJennifer H Mansfield
Mar 24, 2021·Nature Reviews. Molecular Cell Biology·Paul Cohen, Shingo Kajimura
May 1, 2021·Frontiers in Endocrinology·Kasiphak KaikaewJenny A Visser
May 7, 2021·Frontiers in Endocrinology·Abhijit Babaji ShindeQiong A Wang

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.