Ebola virus VP40-induced particle formation and association with the lipid bilayer

Journal of Virology
Luke D JasenoskyYoshihiro Kawaoka

Abstract

Viral protein 40 (VP40) of Ebola virus appears equivalent to matrix proteins of other viruses, yet little is known about its role in the viral life cycle. To elucidate the functions of VP40, we investigated its ability to induce the formation of membrane-bound particles when it was expressed apart from other viral proteins. We found that VP40 is indeed able to induce particle formation when it is expressed in mammalian cells, and this process appeared to rely on a conserved N-terminal PPXY motif, as mutation or loss of this motif resulted in markedly reduced particle formation. These findings demonstrate that VP40 alone possesses the information necessary to induce particle formation, and this process most likely requires cellular WW domain-containing proteins that interact with the PPXY motif of VP40. The ability of VP40 to bind cellular membranes was also studied. Flotation gradient analysis indicated that VP40 binds to membranes in a hydrophobic manner, as NaCl at 1 M did not release the protein from the lipid bilayer. Triton X-114 phase-partitioning analysis suggested that VP40 possesses only minor features of an integral membrane protein. We confirmed previous findings that truncation of the 50 C-terminal amino acids of VP...Continue Reading

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Citations

Feb 1, 2003·Journal of Molecular Biology·Joanna TimminsWinfried Weissenhorn
Mar 13, 2002·Antiviral Research·Mike Bray, Jason Paragas
Oct 13, 2001·Trends in Microbiology·A Takada, Y Kawaoka
Jun 6, 2003·Microbes and Infection·M Javad AmanSina Bavari
Jan 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Peter HalfmannYoshihiro Kawaoka
Sep 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Derek DubeJudith M White
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tanmay A M BharatJohn A G Briggs
Aug 13, 2010·Molecular & Cellular Proteomics : MCP·Kevin B SpurgersSina Bavari
Jul 14, 2004·The Journal of Experimental Medicine·Kelly L WarfieldSina Bavari
Dec 6, 2007·The Journal of Infectious Diseases·Shinji WatanabeYoshihiro Kawaoka
Dec 6, 2007·The Journal of Infectious Diseases·Seiya Yamayoshi, Yoshihiro Kawaoka
Dec 6, 2007·The Journal of Infectious Diseases·Kelly L WarfieldSina Bavari
Oct 19, 2011·The Journal of Infectious Diseases·Yuliang LiuRonald N Harty
Oct 19, 2011·The Journal of Infectious Diseases·Osvaldo MartinezChristopher F Basler
Nov 15, 2011·The Journal of Infectious Diseases·Annika KühlStefan Pöhlmann
Oct 19, 2011·The Journal of Infectious Diseases·Ayaka IwasaYoshihiro Kawaoka
Oct 19, 2011·The Journal of Infectious Diseases·Kelly L Warfield, M Javad Aman
Feb 19, 2010·Journal of Virology·Seiya YamayoshiYoshihiro Kawaoka
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Jan 21, 2011·Journal of Virology·Olena ShtankoYoshihiro Kawaoka
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Apr 23, 2002·Journal of Virology·Takeshi NodaYoshihiro Kawaoka
Jun 14, 2003·Journal of Virology·Ayato TakadaYoshihiro Kawaoka
Aug 28, 2003·Journal of Virology·Jiro YasudaHisatoshi Shida
Mar 29, 2003·Journal of Virology·Michael J BrignatiRichard J Courtney

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