Ebp1 is a dsRNA-binding protein associated with ribosomes that modulates eIF2alpha phosphorylation.

Biochemical and Biophysical Research Communications
Massimo SquatritoGiulio F Draetta

Abstract

dsRNA-binding domains (dsRBDs) characterize an expanding family of proteins involved in different cellular processes, ranging from RNA editing and processing to translational control. Here we present evidence that Ebp1, a cell growth regulating protein that is part of ribonucleoprotein (RNP) complexes, contains a dsRBD and that this domain mediates its interaction with dsRNA. Deletion of Ebp1's dsRBD impairs its localization to the nucleolus and its ability to form RNP complexes. We show that in the cytoplasm, Ebp1 is associated with mature ribosomes and that it is able to inhibit the phosphorylation of serine 51 in the eukaryotic initiation factor 2 alpha (eIF2alpha). In response to various cellular stress, eIF2alpha is phosphorylated by distinct protein kinases (PKR, PERK, GCN2, and HRI), and this event results in protein translation shut-down. Ebp1 overexpression in HeLa cells is able to protect eIF2alpha from phosphorylation at steady state and also in response to various treatments. We demonstrate that Ebp1 interacts with and is phosphorylated by the PKR protein kinase. Our results demonstrate that Ebp1 is a new dsRNA-binding protein that acts as a cellular inhibitor of eIF2alpha phosphorylation suggesting that it could be...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A DasN K Gupta
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H W ChangB L Jacobs
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D St JohnstonM Jantsch
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B DattaN K Gupta
Oct 3, 1996·Biochemical and Biophysical Research Communications·X Li, Y H Chang
Jul 1, 1997·The International Journal of Biochemistry & Cell Biology·M J Clemens
Jun 19, 2001·Trends in Biochemical Sciences·F EisenhaberG Kreil
Mar 4, 2003·Nature Reviews. Cancer·Davide Ruggero, Pier Paolo Pandolfi
Apr 1, 2003·Journal of Cell Science·Joana M P DesterroMaria Carmo-Fonseca
Apr 24, 2003·Science's STKE : Signal Transduction Knowledge Environment·Paul Lasko
May 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura R Saunders, Glen N Barber
Sep 6, 2003·Gene·Micheline Fromont-RacineFranco Fasiolo
Oct 25, 2003·Nucleic Acids Research·Isabel SchlosserDirk Eick
Oct 13, 2004·Nucleic Acids Research·Dragan MarinkovicThomas Wirth
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·Bin TianMichael B Mathews
Jan 5, 2006·Nucleic Acids Research·Carine BarreauH Beverley Osborne

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Citations

Feb 4, 2010·Archives of Virology·M GopinathM S Shaila
Apr 10, 2010·Breast Cancer Research and Treatment·Yan LuAnne W Hamburger
Apr 12, 2008·Cancer Gene Therapy·G Sithanandam, L M Anderson
Jan 27, 2010·Nucleic Acids Research·Juliane MerlHerbert Tschochner
Sep 5, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Eva KowalinskiIrmgard Sinning
Dec 20, 2008·BMC Cell Biology·Yuexing ZhangAnne W Hamburger
Jan 15, 2014·PLoS Neglected Tropical Diseases·Brianna Norris-MullinsMiguel A Morales
Dec 18, 2012·Oncology Reports·Arundhati GhoshAnne W Hamburger
Feb 26, 2014·Journal of Experimental Botany·Rossana HenriquesZoltán Magyar
Jun 18, 2015·International Journal of Oncology·Laura PisapiaGiovanna Del Pozzo
Jun 20, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chen GongChunhua Wan
Oct 7, 2006·The EMBO Journal·Beatrix M HorváthLászló Bögre
Apr 17, 2015·Molecular and Cellular Biochemistry·Smita AwasthiAnne W Hamburger
May 18, 2016·Plant Science : an International Journal of Experimental Plant Biology·Jingtao LiHongyu Pan
Dec 3, 2014·Biochimie·Carmela GiglioneThierry Meinnel
Jan 20, 2010·The International Journal of Biochemistry & Cell Biology·Barbara MichalecMarek Tchórzewski
Jun 28, 2016·American Journal of Human Genetics·Hemanth TummalaTom Vulliamy
Mar 25, 2011·Proteomics·Julie CatusseDominique Job
Aug 5, 2018·Journal of Cellular Physiology·Dang Quan NguyenLe Xuan Truong Nguyen
Aug 11, 2007·The EMBO Journal·Tom P MonieStephen Curry
May 3, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Søren KristiansenMichael Givskov
Mar 29, 2019·Journal of Experimental Botany·Zaki AhmadCsaba Papdi
Jan 31, 2013·British Journal of Cancer·A GhoshA W Hamburger
Jul 6, 2014·Traffic·Hicham Mahboubi, Ursula Stochaj
Jul 29, 2020·Experimental & Molecular Medicine·Inwoo HwangJee-Yin Ahn
May 1, 2016·International Journal of Cancer. Journal International Du Cancer·Hyo Rim KoJee-Yin Ahn
May 10, 2017·RNA·Birgit RogellMatthias W Hentze
Sep 22, 2020·The Journal of Biological Chemistry·Brendan W StevensonJessica K Holien
Aug 19, 2009·Journal of Proteome Research·Kelly A DoroshenkThomas W Okita
Sep 2, 2010·Journal of Proteome Research·Arielis Rodríguez-UlloaSilvio E Perea
Dec 1, 2007·Journal of Proteome Research·Hossein BaharvandGhasem Hosseini Salekdeh

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