EC300: a phage-based, bacteriolysin-like protein with enhanced antibacterial activity against Enterococcus faecalis

Applied Microbiology and Biotechnology
Daniela ProençaCarlos São-José

Abstract

Bacteriophage lytic enzymes, either endolysins or virion-associated lysins, have been receiving considerable attention as potential antibacterial agents, particularly for the combat of antibiotic-resistant Gram-positive pathogens. A conclusion that easily emerges from the careful analysis of a great number of reports on the field is that the activity of phage lytic enzymes is rarely studied in conditions that support robust growth of the target bacteria. Here, we report the construction and study of a chimerical lysin, EC300, which was designed to target and kill Enterococcus faecalis in conditions supporting vigorous bacterial growth. EC300 resulted from the fusion of a predicted M23 endopeptidase domain of a virion-associated lysin to the putative cell wall binding domain of a previously characterized amidase endolysin, both produced by the E. faecalis phage F170/08. This bacteriolysin-like protein exhibited a clear enhanced lytic activity over the parental endolysin when both were assayed in a rich bacterial growth medium. We demonstrate the killing efficacy of EC300 against growing cells of a panel of typed E. faecalis clinical strains with high level of antibiotic resistance. The possible reasons for the marked difference ...Continue Reading

References

Sep 16, 1991·The American Journal of Medicine·D R SchabergR P Gaynes
Aug 1, 1991·Antimicrobial Agents and Chemotherapy·M M HuyckeM S Gilmore
Jan 1, 1990·Clinical Microbiology Reviews·B E Murray
Sep 1, 1989·Antimicrobial Agents and Chemotherapy·D F SahmB Clarke
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C T ChungR H Miller
Oct 1, 1993·Clinical Microbiology Reviews·T G Emori, R P Gaynes
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 13, 1998·The Journal of Antimicrobial Chemotherapy·E SeverinaA Tomasz
Oct 24, 2003·Infection and Immunity·Jutta M LoefflerVincent A Fischetti
Nov 5, 2003·International Journal of Food Microbiology·Günter Klein
Sep 8, 2004·Biochemistry·Hester Emilie HasperEefjan Breukink
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Marian Takác, Udo Bläsi
Jun 28, 2005·Nucleic Acids Research·John Besemer, Mark Borodovsky
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
Apr 7, 2006·Applied and Environmental Microbiology·David M DonovanDavid G Pritchard
Nov 6, 2007·Journal of Bacteriology·João Gil NascimentoMário Almeida Santos
Feb 19, 2008·FEMS Immunology and Medical Microbiology·Janetta TopMarc Bonten
May 6, 2008·Applied and Environmental Microbiology·Jumpei UchiyamaShigenobu Matsuzaki
Jul 9, 2008·Applied Microbiology and Biotechnology·Jaspal K Kumar
Oct 14, 2008·Expert Review of Anti-infective Therapy·Cesar A Arias, Barbara E Murray
Oct 23, 2008·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Hideki NoguchiTakehiko Itoh
Dec 11, 2008·Methods in Molecular Biology·Andrew M KropinskiRoger P Johnson
Feb 3, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R MatoI Santos-Sanches
Apr 23, 2009·Microbiology·Katie Fisher, Carol Phillips
Jul 8, 2009·Journal of Bacteriology·Maria João FriasMário Ramirez
Jun 12, 2010·International Journal of Food Microbiology·Pilar GarcíaAna Rodríguez
Jun 24, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I G SavaJ Huebner
Nov 27, 2010·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Dec 7, 2010·International Journal of Antimicrobial Agents·G L French
Feb 18, 2011·Bioengineered Bugs·Mark FentonAidan Coffey
Jun 21, 2011·Bacteriophage·Stephen T Abedon

❮ Previous
Next ❯

Citations

Mar 25, 2017·Applied Microbiology and Biotechnology·Agnieszka LatkaZuzanna Drulis-Kawa
Jun 2, 2020·Frontiers in Microbiology·Varun Rakeshbhai Bavda, Vikas Jain
Jun 16, 2017·Annual Review of Microbiology·Brittany B Lewis, Eric G Pamer
Jun 9, 2017·Annual Review of Chemical and Biomolecular Engineering·Xia WuJonathan S Dordick
Aug 23, 2019·Biotechnology and Bioengineering·Xia WuJonathan S Dordick
Sep 19, 2020·Applied Microbiology and Biotechnology·Amala BhagwatJonathan S Dordick
Mar 3, 2021·Journal of Biotechnology·Amala BhagwatJonathan S Dordick

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.