PMID: 42174Nov 1, 1979

Ecdysone 3-epimerase from the midgut of Manduca sexta (L.)

R T MayerJ A Svoboda


Ecdysone 3-epimerase was partially purified by ammonium sulfate fractionation from the 100,000 g supernate of Manduca sexta midguts. The enzyme converts ecdysone and 20-hydroxyecdysone to their respective 3-epimers, requires NADH or NADPH and O2 for this reaction, and has the following kinetic parameters: for ecdysone, Km = 17.0 +/- 1.4 microM, Vmax = 110.6 +/- 14.6 pmol min-1 mg-1; for 20-hydroxyecdysone, Km = 47.3 +/- 7.5 microM, Vmax = 131.0 +/- 3.5 pmol min-1 mg-1: for NADPH, Km = 85.4 +/- 10.6 microM; for NADH, Km = 51.3 +/- 1.3 microM. The reaction is irreversible and can be inhibited by various ecdysteroids.


Sep 1, 1978·European Journal of Biochemistry·J Koolman, P Karlson
Oct 1, 1978·Hoppe-Seyler's Zeitschrift für physiologische Chemie·R T MayerG F Weirich
Jan 1, 1977·Journal of Insect Physiology·K D SpindlerH Emmerich
Oct 1, 1977·Hoppe-Seyler's Zeitschrift für physiologische Chemie·J Koolman, K D Spindler
Sep 1, 1974·Steroids·M J ThompsonH N Nigg
Dec 1, 1974·Lipids·H N NiggS R Dutky
Sep 18, 1999·Free Radical Biology & Medicine·H Wang, J A Joseph

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