Ecdysone-induced accumulation of mosquito cells in the G1 phase of the cell cycle

Journal of Insect Physiology
Anna Gerenday, Ann M Fallon

Abstract

We have established baseline conditions for investigating the interaction of the insect steroid hormone 20-hydroxyecdysone (20E) with the cell cycle in the C7-10 cell line from the mosquito, Aedes albopictus. As is the case with Drosophila melanogaster cells, treatment of C7-10 cells with 20E inhibits proliferation. In the presence of 10(-6) M 20E, a gradual decline in cell number is typically apparent at 24 h. Media components such as phenol red and the potential presence of endogenous steroids in serum have no effect on the response to 20E. Pre-treating the cells with 10(-8) M 20E, with or without an intervening hormone-free period, did not alter the response to 10(-6) M 20E. However, replenishment of the medium appeared to synchronize the response to 10(-6) M 20E, causing an abrupt and complete cessation of cell division by 48 h. Flow cytometry over a 20 h period showed a decrease in the proportion of cells in S within 4-6 h after exposure to 20E. By 6-10 h, a transient increase in G2 was followed by the accumulation of more than 70% of the cells in G1. These data suggest that after treatment with 20E, cells complete the ongoing cycle before arresting in G1. Consistent with the decrease in the proportion of cells in S and G2...Continue Reading

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Citations

Oct 13, 2011·PloS One·Maeva GiraudoGaëlle Le Goff
Aug 12, 2009·Cellular and Molecular Life Sciences : CMLS·Klaus-Dieter SpindlerM Spindler-Barth
Feb 6, 2015·International Journal of Molecular Sciences·Wenliang QianDaojun Cheng
Mar 23, 2010·Journal of Insect Physiology·Ann M Fallon, Anna Gerenday
Aug 28, 2009·Archives of Insect Biochemistry and Physiology·Katarzyna BetanskaKlaus-Dieter Spindler
Sep 20, 2011·Archives of Insect Biochemistry and Physiology·Anna Gerenday, Ann M Fallon
Jun 18, 2020·Frontiers in Cell and Developmental Biology·Dani RenWei Guo

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