PMID: 297Jul 1, 1975

Ecdysone Oxidase, an enzyme from the blowfly Calliphora erythrocephala (Meigen)

Hoppe-Seyler's Zeitschrift für physiologische Chemie
J Koolman, P Karlson

Abstract

In the blowfly, the formation of 3-dehydroecdysone from the insect molting hormone ecdysone is catalyzed by an enzyme which carries hydrogen from ecdysone and ecdysterone to oxygen. The enzyme is therefore called "ecdysone oxidase". Two methods are described for the detection of ecdysone oxidase activity, one using a radiolabelled substrate which is separated from the product by thin-layer chromatography after the reaction, and the other using dichloroindophenol, which is discoloured by the redox reaction. The ecdysone oxidase is purified by a factor of 2200 from prepupae of Calliphora erythrocephala using salt precipitation and ion exchange chromatography. The ecdysone oxidase has a Km value for ecdysone of 42muM. The pH optimum is 6.5. The temperature optimum lies at 45 degrees C. The ecdysone oxidase has a molecular weight of 240000.

Citations

Jan 1, 1977·Journal of Insect Physiology·K D SpindlerH Emmerich
Sep 1, 1978·European Journal of Biochemistry·J Koolman, P Karlson
Jun 17, 2011·Archives of Insect Biochemistry and Physiology·Hua-Jun YangYun-Gen Miao
Jul 1, 1976·General and Comparative Endocrinology·R FeyereisenJ A Hoffmann
Feb 14, 1990·Biochemical and Biophysical Research Communications·J SrivatsanM Agosin
Jun 5, 2015·Proceedings. Biological Sciences·Zhiqian LiAnjiang Tan
Oct 1, 1977·Hoppe-Seyler's Zeitschrift für physiologische Chemie·J Koolman, K D Spindler

Related Concepts

Metazoa
Ion-Exchange Chromatography Procedure
Thin Layer Chromatography
Diptera
Ecdysone
Ecdysterone
Hydrogen-Ion Concentration
Oxidation-Reduction
Oxidase
Sodium 2,6-Dichloroindophenol

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