Echinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria

Mycoses
Ulrike KlotzJoerg Steinmann

Abstract

Echinocandin resistance in Candida glabrata is emerging and is associated with the presence of FKS mutations. In this study, we analysed the antifungal susceptibility, presence of FKS mutations and clonality of C. glabrata blood culture isolates from two hospitals in Germany and Austria. Susceptibility testing of 64 C. glabrata bloodstream isolates from two university hospitals was performed with broth microdilution method according to EUCAST. In addition, all isolates were screened for FKS mutations. Molecular fingerprinting was performed by microsatellite PCR with three separate primer pairs and semiautomated repetitive sequenced-based PCR (rep-PCR). One C. glabrata isolate from Germany (1.5%) was echinocandin resistant, with a corresponding mutation in FKS2 gene hot spot 1. The discriminatory power of microsatellite PCR was higher than that of rep-PCR (Simpson Index of 0.94 vs. 0.88); microsatellite PCR created 31 separate genotypes, whereas rep-PCR created 17. Predominant genotypes or clusters of isolates from Germany and Austria were present, with no epidemiological evidence of nosocomial transmissions. Although we found a low incidence of echinocandin resistance in C. glabrata in our settings, further surveillance project...Continue Reading

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Mar 28, 2018·Antimicrobial Agents and Chemotherapy·Xin HouYing-Chun Xu
Nov 7, 2018·Antimicrobial Agents and Chemotherapy·María Ángeles Bordallo-CardonaJesús Guinea
Mar 3, 2019·Mycoses·Hedda Luise VerhasseltJoerg Steinmann
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Aug 12, 2021·JAC-antimicrobial Resistance·Alexander Maximilian AldejohannOliver Kurzai

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