PMID: 8966993Jun 1, 1996Paper

Echinococcus granulosus: genomic and isoenzymatic study of Spanish strains isolated from different intermediate hosts

Veterinary Parasitology
M Siles-LucasC Cuesta-Bandera

Abstract

The phenomenon on intraspecific variation in Echinococcus granulosus is already documented in Spain, where unilocular hydatidosis is an endemic disease. The first speciation studies, focused at a genomic level, showed the existence of three different strains: ovine-bovine-human, equine and swine-caprine. In the present study, the genomic identification, by random amplified polymorphic DNA technique (RAPD) of a larger number of Spanish E. granulosus isolates, using five different primers, showed the maintenance of these groups. Thus, some of these strains may not be infective for man. These conclusions were supported by a phenotypic characterization of the same isolates by zymodeme technique, showing the five isoenzyme systems used that Spanish E. granulosus strains can also be distinguished at a phenotypic level by isoenzymatic patterns. Both techniques (RAPD and zymodemes) were used for statistical analysis and for the construction of two dendrograms, which were slightly different. In addition, some intrastrain variation was detected with both techniques, a phenomenon that is directly related to the different speciation theories proposed for E. granulosus strains. The epidemiological implications of the results are discussed i...Continue Reading

References

Jan 1, 1979·Transactions of the Royal Society of Tropical Medicine and Hygiene·D P McManus, J D Smyth
Apr 1, 1991·International Journal for Parasitology·A J Lymbery, R C Thompson
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Dec 1, 1989·International Journal for Parasitology·A J Lymbery, R C Thompson
Nov 1, 1985·Molecular and Biochemical Parasitology·D P McManus, A J Simpson
Sep 1, 1988·International Journal for Parasitology·A J Lymbery, R C Thompson
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·K MullisH Erlich
Jan 1, 1986·Zeitschrift Für Parasitenkunde·L M KumaratilakeA D'Alessandro
Jan 1, 1969·Journal of Helminthology·J D Smyth, M M Smyth
Jan 1, 1993·Parasitology Research·M Siles-LucasM César-Benito
Jan 1, 1993·Molecular and Biochemical Parasitology·E Dias NetoA J Simpson
Mar 1, 1993·The Journal of Eukaryotic Microbiology·M F DirieP R Gardiner

❮ Previous
Next ❯

Citations

Mar 5, 2003·Experimental Parasitology·L M GonzálezC Cuesta-Bandera
Jul 8, 1998·Veterinary Parasitology·F Ponce Gordo, C Cuesta Bandera
Mar 1, 1997·Journal of Helminthology·F Ponce Gordo, C Cuesta Bandera
Sep 30, 2000·Parasite Immunology·M Siles-LucasM Breijo
Jan 26, 2013·PLoS Neglected Tropical Diseases·Ghalia BoubakerMarkus Spiliotis
Apr 25, 2008·Parasitology International·Carlos ManterolaJuan Carlos Roa
Mar 31, 2004·Memórias do Instituto Oswaldo Cruz·Pablo MaravillaAna Flisser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.