Echinococcus granulosus in Spain: strain differentiation by SDS-PAGE of somatic and excretory/secretory proteins

Journal of Helminthology
M Siles-Lucas, C Cuesta-Bandera

Abstract

A comparison was made, by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), of excretory/secretory (ES)-crude and immunopurified (with the corresponding anti-host serum) hydatid fluids-and somatic (S)-protoscoleces-proteins, from several ovine, equine, swine, bovine and human Echinococcus granulosus Spanish isolates. Likewise, the host influence on parasitic ES protein expression was studied, comparing purified hydatid fluids from ovine and equine cysts obtained from natural hosts and in RNMI mice. Purified hydatid fluids patterns, under reducing conditions, yielded the most precise differentiation of Spanish strains of E. granulosus into three groups (ovine-bovine-human, equine and swine), the finding of a characteristic 82 kDa band in equine isolates, and an unusual arrangement of bands between 50 and 6 kDa in swine samples. In addition, differences were found amongst crude and purified hydatid fluids, especially in bovine and swine isolates. The total protein patterns of protoscoleces were most complex, and therefore could not be used for strain differentiation. Finally, the purified hydatid fluids from cysts developed in natural and experimental hosts showed similar protein patterns, suggesting the lack ...Continue Reading

Citations

Jan 1, 1988·Parasitology Research·J M Abdul-Salam, M A Farah
Jul 8, 1998·Veterinary Parasitology·F Ponce Gordo, C Cuesta Bandera
Nov 27, 2015·Tropical Medicine & International Health : TM & IH·Marcela Alejandra CucherLaura Kamenetzky
Sep 11, 2007·Experimental Parasitology·Patricia Marcela MuzulinMara Cecilia Rosenzvit
Jul 6, 2004·Acta Tropica·K Daniel MwambeteC Cuesta-Bandera
Sep 8, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Said ShalabyNeelima Gupta

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