Echinococcus multilocularis primary cells: improved isolation, small-scale cultivation and RNA interference

Molecular and Biochemical Parasitology
Markus SpiliotisBruno Gottstein

Abstract

In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time. This allows assessments on the effects of RNA interference performed on Echinococcus primary cells with regard to growth, proliferation, differentiation of the parasite and the formation of novel metacestode vesicles in vitro.

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Citations

May 4, 2013·International Journal for Parasitology·Natalia N Pouchkina-StantchevaPeter D Olson
Aug 23, 2011·Parasitology·Sutas SuttiprapaPaul J Brindley
Jun 6, 2014·PLoS Neglected Tropical Diseases·Andreas SchubertKlaus Brehm
Aug 25, 2015·Veterinary Parasitology·U Koziol, K Brehm
Jun 30, 2011·Parasite Immunology·J HagenB H Kalinna
Sep 22, 2015·SpringerPlus·Bárbara MoguelJuan P Laclette
Jun 10, 2015·Parasites & Vectors·Dandan HuGuangyou Yang
Feb 25, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Raúl J BobesJuan P Laclette
Oct 14, 2014·Molecular and Biochemical Parasitology·Nicolás Dell'OcaJose F Tort
Feb 11, 2015·International Journal for Parasitology·Marcela CucherMara Cecilia Rosenzvit
Dec 20, 2014·Parasite : Journal De La Société Française De Parasitologie·Andrew HemphillBruno Gottstein
Dec 20, 2014·Parasite : Journal De La Société Française De Parasitologie·Klaus Brehm, Uriel Koziol
Mar 12, 2016·PLoS Neglected Tropical Diseases·Britta StadelmannAndrew Hemphill
May 19, 2017·PLoS Neglected Tropical Diseases·Dominic RitlerBritta Lundström-Stadelmann
Nov 8, 2017·World Journal of Hepatology·Nikica M GruborYehuda Shoenfeld
Mar 15, 2013·Nature·Isheng J TsaiMatthew Berriman
Nov 27, 2018·Parasite : Journal De La Société Française De Parasitologie·Hui WangHao Wen
Dec 28, 2019·PLoS Neglected Tropical Diseases·Matías Gastón PérezMara Cecilia Rosenzvit
Sep 25, 2019·ELife·Tania RozarioPhillip A Newmark
Feb 26, 2020·Food and Waterborne Parasitology·Britta Lundström-StadelmannAndrew Hemphill
Jan 8, 2021·Parasites & Vectors·Michaela Herz, Klaus Brehm
Feb 25, 2019·Molecular and Biochemical Parasitology·T BasikaH B Ferreira

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