Echinoid regulates Flamingo endocytosis to control ommatidial rotation in the Drosophila eye

Development
Yu-Huei HoJui-Chou Hsu

Abstract

Planar cell polarity (PCP) refers to a second polarity axis orthogonal to the apicobasal axis in the plane of the epithelium. The molecular link between apicobasal polarity and PCP is largely unknown. During Drosophila eye development, differentiated photoreceptors form clusters that rotate independently of the surrounding interommatidial cells (ICs). Here, we demonstrate that both Echinoid (Ed), an adherens junction-associated cell adhesion molecule, and Flamingo (Fmi), a PCP determinant, are endocytosed via a clathrin-mediated pathway in ICs. Interestingly, we found that Ed binds the AP-2 adaptor and is required for the internalization of Fmi into ICs. Loss of ed led to increased amounts of Fmi on the cell membrane of non-rotating ICs and also to the misrotation of photoreceptor clusters. Importantly, overexpression of fmi in ICs alone was sufficient to cause misrotation of the adjacent photoreceptor clusters. Together, we propose that Ed, when internalized by AP-2, undergoes co-endocytosis with, and thereby decreases, Fmi levels on non-rotating ICs to permit correct rotation of ommatidial clusters. Thus, co-endocytosis of Ed and Fmi provides a link between apicobasal polarity and PCP.

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Citations

Nov 1, 2011·Molecular and Cellular Biology·Avais M DaulatStéphane Angers
May 2, 2012·Journal of Biomedical Science·Chiao-Ming LinJui-Chou Hsu
Aug 22, 2012·Annual Review of Cell and Developmental Biology·John B Wallingford
Jan 26, 2011·The Journal of Cell Biology·Caroline Laplante, Laura A Nilson
Mar 5, 2016·Cellular and Molecular Life Sciences : CMLS·Marianne Malartre
Jun 7, 2015·Biochemical and Biophysical Research Communications·Yu-Chiao LiJui-Chou Hsu
Apr 23, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Meagan ValentineSimon Collier

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