ECIS based wounding and reorganization of cardiomyocytes and fibroblasts in co-cultures

Progress in Biophysics and Molecular Biology
F Cavallini, M Tarantola

Abstract

The crosstalk of two major heart cell groups, cardiomyocytes and fibroblasts, relies on direct electromechanical cellular coupling as well as indirect mechanical signal transmission through the surrounding viscoelastic extracellular matrix. Upon injury of cardiac tissue, this communication becomes unbalanced: fibrosis is initiated leading to increased collagen deposition, accompanied by an activation of fibroblasts - the key players of fibrosis. They undergo a reorganization or partial transformation to myofibroblasts during this process, which precedes scar formation within the infarcted heart in vivo. Here, we induce wound formation in an in vitro system as a model for these fibrotic conditions: we assessed the dynamics of wound healing in co-cultures of fibroblasts and myocytes upon targeted wound initiation using Electric Cell Substrate Impedance Sensing (ECIS) under optical control. We discovered distinct wound closure dynamics for mono- and co-cultures of myocytes and fibroblasts and observed a cessation of the contractile behavior for recovering cardiomyocyte cultures. We furthermore identified a change of cellular impedance for recovering fibroblasts and the presence of α-SMA, suggesting a partial transformation into my...Continue Reading

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Citations

May 16, 2020·Frontiers in Bioengineering and Biotechnology·Ravikumar B ShindePandiaraj Manickam
Oct 15, 2020·Journal of Wound Care·Seyedeh-Sara HashemiMehrzad Jafari Barmak
Aug 19, 2021·American Journal of Physiology. Cell Physiology·Kyle H MooreEric M George

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