ECM-based microfluidic gradient generator for tunable surface environment by interstitial flow.

Biomicrofluidics
Azusa ShimizuHiroaki Onoe

Abstract

We present an extracellular matrix (ECM)-based gradient generator that provides a culture surface with continuous chemical concentration gradients created by interstitial flow. The gelatin-based microchannels harboring gradient generators and in-channel micromixers were rapidly fabricated by sacrificial molding of a 3D-printed water-soluble sacrificial mold. When fluorescent dye solutions were introduced into the channel, the micromixers enhanced mixing of two solutions joined at the junction. Moreover, the concentration gradients generated in the channel diffused to the culture surface of the device through the interstitial space facilitated by the porous nature of the ECM. To check the functionality of the gradient generator for investigating cellular responses to chemical factors, we demonstrated that human umbilical vein endothelial cells cultured on the surface shrunk in response to the concentration gradient of histamine generated by interstitial flow from the microchannel. We believe that our device could be useful for the basic biological study of the cellular response to chemical stimuli and for the in vitro platform in drug testing.

References

Sep 1, 1969·The Journal of Cell Biology·G MajnoM Leventhal
Jan 28, 1983·European Journal of Pharmacology·J Van de Voorde, I Leusen
Jul 1, 1995·Physiological Reviews·P F Davies
Jan 26, 2002·Science·Abraham D StroockGeorge M Whitesides
Sep 24, 2004·Experimental Cell Research·Shur-Jen WangNoo Li Jeon
Oct 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Cara-Lynn E HelmMelody A Swartz
Apr 19, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Rico C GunawanDeborah E Leckband
May 13, 2006·Analytical Chemistry·Daniel IrimiaMehmet Toner
Jun 2, 2006·Lab on a Chip·Bong Geun ChungNoo Li Jeon
Dec 5, 2006·Trends in Cell Biology·Joseph M Rutkowski, Melody A Swartz
Apr 27, 2007·Annual Review of Biomedical Engineering·Melody A Swartz, Mark E Fleury
Dec 21, 2007·Lab on a Chip·Thomas M Keenan, Albert Folch
Jan 4, 2008·Nature Reviews. Drug Discovery·Robin L ThurmondPaul J Dunford
May 5, 2009·Applied and Environmental Microbiology·Derek L EnglertArul Jayaraman
Jul 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Annarita Di LorenzoWilliam C Sessa
Aug 26, 2009·Lab on a Chip·Javier AtenciaLaurie E Locascio
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Fei Wang
Feb 4, 2010·BMC Biology·Jaime MillánAnne J Ridley
Aug 25, 2010·Electrophoresis·Bong Geun Chung, Jaebum Choo
Oct 20, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Sudong KimNoo Li Jeon
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·William J PolacheckRoger D Kamm
Mar 7, 2012·The Journal of Cell Biology·Stephan HuveneersJohan de Rooij
Nov 10, 2013·Nature Medicine·Daniela F Quail, Johanna A Joyce
Jan 15, 2014·Biochimica Et Biophysica Acta·Francesca GattazzoPaolo Bonaldo
Mar 7, 2014·Cell and Tissue Research·Lucie Duluc, Beata Wojciak-Stothard
Apr 11, 2015·Nature Communications·Constantinos M MikelisJ Silvio Gutkind
Nov 15, 2018·Micromachines·Wei Huang Goh, Michinao Hashimoto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.