ECM production of primary human and bovine chondrocytes in hybrid PEG hydrogels containing type I collagen and hyaluronic acid.

Biomacromolecules
Laura A Smith CallahanMatthew L Becker

Abstract

The development of advanced materials that facilitate hyaline cartilage formation and regeneration in aging populations is imperative. Critical to the success of this endeavor is the optimization of ECM production from clinically relevant cells. However, much of the current literature focuses on the investigation of primary bovine chondrocytes from young calves, which differ significantly than osteoarthritic cells from human sources. This study examines the levels of extracellular matrix (ECM) production using various levels of type I collagen and hyaluronic acid in poly(ethylene glycol) dimethacrylate (PEGDM) hydrogels in total knee arthroplasties, compared with the results from bovine chondrocytes. The addition of type 1 collagen in both the presence and absence of low levels of hyaluronic acid increased ECM production and/or retention in scaffolds containing either bovine or human chondrocytes. These findings are supported consistently with colorimetric quantification, whole mount extracellular matrix staining for both cell types, and histological staining for glycoaminoglycans and collagen of human chondrocyte containing samples. While exhibiting similar trends, the relative ECM productions levels for the primary human chon...Continue Reading

References

Oct 1, 1989·Journal of Biomedical Materials Research·T Canal, N A Peppas
Apr 1, 1994·The Journal of Clinical Investigation·A P HollanderA R Poole
Jul 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J A MartinJ A Buckwalter
Jul 9, 1999·Journal of Biomedical Materials Research·X Wei, K Messner
May 29, 2000·Journal of Biomedical Materials Research·J ElisseeffR Langer
Jul 17, 2001·Anatomy and Embryology·A J HayesC W Archer
May 2, 2003·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·J Richard SteadmanWilliam G Rodkey
Nov 10, 2005·Arthritis Research & Therapy·E N Blaney DavidsonW B van den Berg
Dec 31, 2005·Arthritis and Rheumatism·Jennifer M Hootman, Charles G Helmick
Nov 7, 2006·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Peter C KreuzNorbert Südkamp
Aug 19, 2007·FEBS Letters·Nathaniel S HwangJennifer Elisseeff
Jan 1, 2008·Arthritis and Rheumatism·Reva C LawrenceUNKNOWN National Arthritis Data Workgroup
Oct 31, 2008·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Mehmet AsikAtacan Atalar
Feb 28, 2009·Methods in Molecular Biology·Iossif A Strehin, Jennifer H Elisseeff
Feb 5, 2010·Best Practice & Research. Clinical Rheumatology·A Shane Anderson, Richard F Loeser
Oct 1, 2010·Stem Cells·Anthony P HollanderWael Kafienah
Feb 19, 2011·Pharmaceutical Research·Susan LinShyni Varghese
Mar 15, 2011·Osteoarthritis and Cartilage·N E LaneM C Hochberg
Apr 20, 2011·Cell and Tissue Research·Nathaniel S HwangJennifer Elisseeff

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Citations

Sep 17, 2013·European Polymer Journal·Tianzhi Luo, Kristi L Kiick
Nov 27, 2014·Biomacromolecules·Hang Kuen Lau, Kristi L Kiick
Sep 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shangwu ChenGuoping Chen
Apr 25, 2021·Acta Biomaterialia·João F Crispim, Keita Ito

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