ECM-Regulator timp Is Required for Stem Cell Niche Organization and Cyst Production in the Drosophila Ovary

PLoS Genetics
John R PearsonAcaimo González-Reyes

Abstract

The extracellular matrix (ECM) is a pivotal component adult tissues and of many tissue-specific stem cell niches. It provides structural support and regulates niche signaling during tissue maintenance and regeneration. In many tissues, ECM remodeling depends on the regulation of MMP (matrix metalloproteinase) activity by inhibitory TIMP (tissue inhibitors of metalloproteinases) proteins. Here, we report that the only Drosophila timp gene is required for maintaining the normal organization and function of the germline stem cell niche in adult females. timp mutant ovaries show reduced levels of both Drosophila Collagen IV α chains. In addition, tissue stiffness and the cellular organization of the ovarian niche are affected in timp mutants. Finally, loss of timp impairs the ability of the germline stem cell niche to generate new cysts. Our results demonstrating a crucial role for timp in tissue organization and gamete production thus provide a link between the regulation of ECM metabolism and tissue homeostasis.

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Citations

Dec 10, 2016·Nature Reviews. Cancer·Hartland W JacksonRama Khokha
Oct 19, 2017·Development·Julien ChlastaMuriel Grammont
Sep 12, 2018·Systems Biology in Reproductive Medicine·Yangchun LiuYuehui Zheng
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Feb 24, 2021·Current Biology : CB·Alfonsa Díaz-TorresAcaimo González-Reyes
Oct 5, 2021·General and Comparative Endocrinology·Yong Zhu

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Methods Mentioned

BETA
transmission electron-microscopy
Electrophoresis
Fluorescence
confocal microscopy
Atomic Force Microscopy
AFM
electron microscopy
dissection
Transmission electron microscopy

Software Mentioned

Mascot
Matlab
ImageJ
GeneCodis
IMARIS
Imaris Measurement
PANTHER

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