Apr 23, 2020

FORCAST: a fully integrated and open source pipeline to design Cas-mediated mutagenesis experiments

BioRxiv : the Preprint Server for Biology
H. ElrickLauryl M. J. Nutter

Abstract

Cas-mediated genome editing has enabled researchers to perform mutagenesis experiments with relative ease. Effective genome editing requires tools for guide RNA selection, off-target prediction, and genotyping assay design. While independent tools exist for these functions, there is still a need for a comprehensive platform to design, view, evaluate, store, and catalogue guides and their associated primers. The Finding Optimizing and Reporting Cas Targets (FORCAST) application integrates existing open source tools such as JBrowse, Primer3, BLAST, bwa, and Silica to create a complete allele design and quality assurance pipeline. FORCAST is a fully integrated software that allows researchers performing Cas-mediated genome editing to generate, visualize, store, and share information related to guides and their associated experimental parameters. It is available from a public GitHub repository and as a Docker image, for ease of installation and portability.

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Mentioned in this Paper

Metabolic Process, Cellular
Research
Virus
Genome
Genes
Genomic Stability
Archaeal Viruses
Sulfur
TRIOBP gene
Genomics

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