Ecological implications of an improved direct viable count method for aquatic bacteria.

Applied and Environmental Microbiology
F Joux, P Lebaron

Abstract

The direct viable count method first described by Kogure et al. (Can. J. Microbiol. 25:415-420, 1979) was improved by using an antibiotic cocktail instead of nalidixic acid alone. We screened 100 marine isolates from two coastal areas for their sensitivities to five replication-inhibiting antibiotics, including four quinolones (nalidixic, piromidic, and pipemidic acids and ciprofloxacin) and one (beta)-lactam (cephalexin). It was shown that growth inhibition of all isolates cannot be readily achieved by using a single antibiotic. Inhibition was much more efficient when all the antibiotics were combined, making it possible to use this method with natural communities. In combination, the concentration of each antibiotic could be lowered and the incubation time could be increased without any growth. Under such conditions, it was shown that the fraction of substrate-responsive cells within natural marine communities is much greater (1 to 2 orders of magnitude) than those reported by traditional procedures. Furthermore, the new procedure made substrate-responsive cells more clearly distinguishable. These improvements resulted in an increased incubation time and were related to metabolic expression of slow-growing cells and/or to the...Continue Reading

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Citations

Jan 20, 2006·FEMS Microbiology Ecology·Patrick K JjembaJodi R Shann
Aug 1, 2002·FEMS Microbiology Ecology·Karine Lemarchand, Philippe Lebaron
Feb 5, 2008·Applied and Environmental Microbiology·Woo Kyung JungYong Ho Park
Mar 4, 2008·Applied and Environmental Microbiology·Poornima GourabathiniSharon G Berk
Jun 17, 2011·The ISME Journal·Satoshi IshiiKeishi Senoo
Jul 2, 2004·Folia Microbiologica·L Lipoglavsek, G Avgustin
Mar 8, 2019·Microbiology·Shravanthi S Kumar, Asit Ranjan Ghosh
Jun 30, 2011·Environmental Monitoring and Assessment·Christabelle E G FernandesP A Loka Bharathi
Jun 8, 2017·Frontiers in Microbiology·Vijayan JasnaTélesphore Sime-Ngando
Mar 24, 2010·Applied Microbiology and Biotechnology·Satoshi IshiiKeishi Senoo
Jun 15, 2010·Applied and Environmental Microbiology·Mohiuddin M Taimur KhanAnne K Camper
Mar 27, 2007·Applied and Environmental Microbiology·Takehiko KenzakaMasao Nasu
Feb 12, 2017·Folia Microbiologica·Itxaso MontánchezVladimir R Kaberdin
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Nov 16, 2010·Applied and Environmental Microbiology·Satoshi IshiiKeishi Senoo
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