Ecto-5'-nucleotidase mediates infarct size-limiting effect by ischemic preconditioning in the rabbit heart

Journal of Cardiovascular Pharmacology
K KomamuraM Hori

Abstract

We examined whether ecto-5'-nucleotidase mediates infarct limitation by ischemic preconditioning in the rabbit heart. Ecto-5'-nucleotidase activity in ischemic region after ischemic preconditioning was greater than that in nonischemic regions (23.6 +/- 2.5 vs. 13.6 +/- 1.0 nmol/mg protein/min; p < 0.01). With an inhibitor of 5'-nucleotidase, alpha,beta-methylene adenosine 5'-diphosphate (AMP-CP), ecto-5'-nucleotidase activity in the ischemic region was comparable to that in the nonischemic region. Mean blood pressure was reduced from 73 +/- 2 to 62 +/- 3 mm Hg with intravenous AMP, whereas it did not change with coperfusion of AMP and AMP-CP, suggesting effective inhibition of ecto-5'-nucleotidase. Separately, myocardial infarction was created by 30-min coronary occlusion and 3 h of reperfusion. Infarct size expressed as percentage volume in risk area was reduced by ischemic preconditioning compared with that in the control (7.8 +/- 2.5% vs. 38.1 +/- 4.0%; p < 0.01). However, infarct size in the group given AMP-CP plus ischemic preconditioning was similar to that in the control (36.2 +/- 2.8% vs. 38.1 +/- 4.0%; NS), suggesting that ecto-5'-nucleotidase mediates infarct limitation by ischemic preconditioning in the rabbit.

References

Jun 1, 1977·The Journal of Physiology·T Forrester, C A Williams
Feb 1, 1992·Circulation Research·G J Gross, J A Auchampach
Mar 1, 1992·Journal of Molecular and Cellular Cardiology·J P HeadrickR M Berne
Jul 15, 1992·The Biochemical Journal·H Zimmermann
Aug 1, 1989·Pflügers Archiv : European journal of physiology·S ImaiM Nakazawa
Nov 1, 1989·Canadian Journal of Physiology and Pharmacology·C A CourneyaJ R Ledsome
Oct 1, 1988·Journal of Molecular and Cellular Cardiology·A A WildeM J Janse
Sep 1, 1966·Cancer·K SmithJ T Mallams
Jan 1, 1994·Advances in Experimental Medicine and Biology·A B AgostiñhoR Leoncini
Jan 1, 1994·Advances in Experimental Medicine and Biology·F RosiE Dispensa
Aug 1, 1994·Journal of Molecular and Cellular Cardiology·M J RaatikainenI E Hassinen
Nov 1, 1994·The American Journal of Physiology·A ChangM L Zeidel
Jan 1, 1995·Circulation Research·M B MitchellA Banerjee
Aug 1, 1993·Circulation·S Ballin, C Louis
Mar 1, 1993·Journal of Molecular and Cellular Cardiology·J D ThorntonJ M Downey
Oct 1, 1995·Journal of Molecular and Cellular Cardiology·M KitakazeT Kamada
Nov 1, 1995·Journal of Molecular and Cellular Cardiology·X ZhaiM Ashraf
Mar 15, 1996·Journal of the American College of Cardiology·S HoshidaM Tada

❮ Previous
Next ❯

Citations

May 13, 2010·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Joanna LeckaMichal Komoszynski
Nov 14, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Y F ChenA P Zou
Oct 19, 2007·American Journal of Physiology. Renal Physiology·Martha FrancoFlavio Martínez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.