Ecto-diadenosine polyphosphates hydrolase activity on human prostasomes

The Prostate
A MinelliG Ronquist

Abstract

Ecto-diadenosine polyphosphates are ubiquitous compounds with several physiological roles. Ecto-diadenosine polyphosphates hydrolase control their actions by degrading and terminating their signaling. The present work deals with the identification and partial characterization of ecto-diadenosine polyphosphates hydrolase on human prostasomes. Reverse-phase and paired-ion HPLC techniques have been used. Prostasomes have an ecto-diadenosine polyphosphates hydrolase that leads to the degradation of several diadenosine compounds. Kinetic parameters of the enzyme show that diadenosine tetraphosphate is the preferred substrate that is further metabolized by the prostasome-ecto-nucleotidases to adenosine. The ecto-enzyme is bound to the prostasome-membranes through a GPI-anchor and is activated by physiological concentration of Ca+2, Mg+2, and Mn+2. Its optimum pH is also in the slightly alkaline physiological range. Human spermatozoa do not possess this hydrolytic activity, but they can acquire it after fusion with prostasomes. The existence of an enzyme capable of degrading diadenosine compounds and can be transferred to human spermatozoa suggests new physiological implications for the role of prostasomes in fertilization.

References

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Citations

Jul 9, 2003·European Journal of Biochemistry·Petra VollmayerHerbert Zimmermann
Feb 1, 2008·Reproductive Biology and Endocrinology : RB&E·Laura SicilianoAmalia Carpino
Oct 24, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Marian AalbertsWillem Stoorvogel
Sep 4, 2012·Biochimica Et Biophysica Acta·Marian AalbertsWillem Stoorvogel
Feb 2, 2018·Endocrine Reviews·Carlos SimonFelipe Vilella
Dec 24, 2005·Human Reproduction Update·H P BurdenK Whittington

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