PMID: 8970811Sep 15, 1996Paper

Ectonucleotidase activity in the perilymphatic compartment of the guinea pig cochlea

Hearing Research
S M VlajkovicG D Housley

Abstract

It has been clearly demonstrated that extracellular adenosine 5'-triphosphate (ATP) exerts a potent modulatory activity in the cochlea through its interaction with P2 purinoceptors. However, little is known regarding the metabolism of extracellular ATP in cochlear tissues via ectonucleotidases. This study provides evidence for the presence of ectonucleotidases in the perilymphatic compartment of the guinea pig cochlea. Using microperfusion, ATP (500 microM) was introduced into the cochlear perilymph through the basal turn scala tympani, and effluent was collected from the basal turn scala vestibuli. Samples were subsequently analysed for the presence of adenine metabolites using high performance liquid chromatography (HPLC). Cell viability was evaluated by the activity of the intracellular enzyme lactate dehydrogenase (LDH) in the perfusate. ATP was degraded to 122.8 +/- 9.9 microM (25.0 +/- 5.8%) during the passage through the cochlear perilymphatic compartment. Breakdown of ATP resulted in the formation of adenosine 5'-diphosphate (41.5 +/- 9.0 microM), adenosine 5'-monophosphate (201.3 +/- 15.5 microM), adenosine (108.6 +/- 8.3) and inosine (15.0 +/- 1.5 microM). The degradation of ATP was significantly (P < 0.001, Student's...Continue Reading

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Citations

Apr 29, 1998·Molecular Neurobiology·G D Housley
Sep 5, 2002·Hearing Research·Srdjan M VlajkovicGary D Housley
Nov 5, 1997·Hearing Research·R A SkellettR P Bobbin
Dec 5, 1997·Hearing Research·R P BobbinM Fallon
Jan 30, 1999·Hearing Research·D J MuñozP R Thorne
Oct 19, 2001·The International Journal of Biochemistry & Cell Biology·V P VieiraM R Schetinger
Nov 6, 2007·Hearing Research·Jun Ho Lee, Daniel C Marcus
Jul 22, 2010·Journal of Neuroscience Research·Srdjan M VlajkovicPeter R Thorne
Jun 15, 2005·Hearing Research·Richard P Bobbin, Sanford C Bledsoe
Nov 6, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Srdjan M VlajkovicGary D Housley
Feb 23, 2007·Neuroreport·Abdul F KhanSrdjan M Vlajkovic
Aug 5, 2019·Purinergic Signalling·Jennie M E CederholmGary D Housley
Aug 5, 1998·The American Journal of Physiology·D ButlenE Ferrary

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