Ectonucleotidases in Müller glial cells of the rodent retina: Involvement in inhibition of osmotic cell swelling.

Purinergic Signalling
Ianors IandievAndreas Bringmann

Abstract

Extracellular nucleotides mediate glia-to-neuron signalling in the retina and are implicated in the volume regulation of retinal glial (Müller) cells under osmotic stress conditions. We investigated the expression and functional role of ectonucleotidases in Müller cells of the rodent retina by cell-swelling experiments, calcium imaging, and immuno- and enzyme histochemistry. The swelling of Müller cells under hypoosmotic stress was inhibited by activation of an autocrine purinergic signalling cascade. This cascade is initiated by exogenous glutamate and involves the consecutive activation of P2Y(1) and adenosine A1 receptors, the action of ectoadenosine 5'-triphosphate (ATP)ases, and a nucleoside-transporter-mediated release of adenosine. Inhibition of ectoapyrases increased the ATP-evoked calcium responses in Müller cell endfeet. Müller cells were immunoreactive for nucleoside triphosphate diphosphohydrolases (NTPDase)2 (but not NTPDase1), ecto-5'-nucleotidase, P2Y(1), and A1 receptors. Enzyme histochemistry revealed that ATP but not adenosine 5'-diphosphate (ADP) is extracellularly metabolised in retinal slices of NTPDase1 knockout mice. NTPDase1 activity and protein is restricted to blood vessels, whereas activity of alkalin...Continue Reading

References

Jul 15, 1992·The Biochemical Journal·H Zimmermann
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N F RebbeS Hickman
Feb 1, 1992·Archives of Ophthalmology·G A Lutty, D S McLeod
Oct 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S T Hussain, E A Baydoun
Feb 1, 1982·Journal of Neurocytology·G W Kreutzberg, S T Hussain
Aug 28, 1995·Brain Research. Developmental Brain Research·N BraunH Zimmermann
Feb 11, 2000·Archives of Oral Biology·F J DowdW Zeng
Apr 15, 2000·The Journal of Biological Chemistry·E BodasC Solsona
Dec 9, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·H Zimmermann
Mar 21, 2001·Nature Reviews. Neuroscience·P G Haydon
Sep 5, 2002·Nature Reviews. Neuroscience·Maiken NedergaardAnker J Hansen
Apr 26, 2003·The European Journal of Neuroscience·Norbert BraunHerbert Zimmermann
Oct 3, 2003·Trends in Neurosciences·Eric A Newman
Dec 4, 2003·Glia·Ortrud UckermannAndreas Bringmann
Jul 28, 2004·Molecular and Cellular Neurosciences·Thomas PannickeAndreas Bringmann
Apr 29, 2005·American Journal of Physiology. Cell Physiology·David ReigadaClaire H Mitchell
Jun 10, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eric A Newman
Oct 25, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D Scott McLeodGerard Lutty
Mar 21, 2007·Neuroscience·T Puthussery, E L Fletcher

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Citations

Apr 8, 2014·Pflügers Archiv : European journal of physiology·Christian LohrDaniela Hirnet
Apr 2, 2016·Journal of Diabetes Research·J VindeirinhoP F Santos
Jun 20, 2008·Pharmacology & Therapeutics·Almudena CrookeJesús Pintor
Jan 1, 2009·Synapse·María Jimena RicattiMaría Paula Faillace
Nov 20, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M A M Willart, B N Lambrecht
May 23, 2015·Neuropharmacology·Andreas Reichenbach, Andreas Bringmann
Dec 17, 2014·Purinergic Signalling·Kristine GampeHerbert Zimmermann
Sep 18, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sirpa LoukovaaraGennady G Yegutkin
Oct 19, 2016·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Andreas Reichenbach, Andreas Bringmann
Sep 17, 2011·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Andreas Bringmann, Peter Wiedemann
Feb 27, 2013·Glia·Andreas Reichenbach, Andreas Bringmann
Jan 9, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Julian ZeinerGennady G Yegutkin
Dec 17, 2009·Journal of Neurochemistry·Antje WurmAndreas Bringmann
Mar 19, 2013·The Journal of Pharmacology and Experimental Therapeutics·Ana Guzman-AranguezJesús Pintor
Jan 10, 2020·Molecular Neurobiology·Rupali Vohra, Miriam Kolko
Jul 6, 2019·Purinergic Signalling·Stephanie L GrilloSalvatore L Stella
Jun 28, 2018·Purinergic Signalling·María J Pérez de LaraJesús Pintor
Feb 29, 2020·Pharmacology & Therapeutics·Ana Raquel SantiagoAntónio Francisco Ambrósio
Dec 31, 2008·Neurochemistry International·Andreas BringmannAndreas Reichenbach
Feb 24, 2009·Trends in Neurosciences·Gary D HousleyAndreas Reichenbach

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Prism
LSM
Graphpad

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