Ectopic bone formation after implantation of a slow release system of polylactic acid and rhBMP-2

Clinical Oral Implants Research
R GruberH Schliephake

Abstract

The present study was conducted to test the hypothesis that preshaped polylactic acid (PLA) implants loaded with recombinant human bone morphogenic protein 2 (rhBMP-2) can induce bone formation in a rat ectopic model. Two groups of porous cylindrical poly-DL-lactic acid implants of 8-mm diameter were produced by gas foaming with CO(2), incorporating 48 and 96 microg rhBMP-2, respectively, into each implant. Blank PLA implants were used as controls. The release of BMPs and the induction of alkaline phosphatase were assessed in vitro. Osteoinduction in vivo was tested by insertion of 15 implants from each group into the gluteal muscles of Wistar rats. Five implants from each group were retrieved after 6, 13 and 26 weeks and assessed using flat panel volume detector computed tomography and light microscopy. Both groups of implants showed increased release of rhBMP-2 during the first 24-48 h, with a slightly higher amount being released from the implants with 48 microg. Release during subsequent intervals was <100 ng/72 h in the low-concentration group and >100 ng in the group with 96 microg rhBMP-2. Implants with 95 microg rhBMP-2 exhibited bone formation in vivo on the outside of the implants across the observation period of 26 w...Continue Reading

References

Dec 31, 1997·The Journal of Bone and Joint Surgery. American Volume·H D ZegzulaJ O Hollinger
Sep 30, 1999·International Journal of Pharmaceutics·W FriessC Sargeant
Nov 5, 1999·Clinical Orthopaedics and Related Research·S R WinnJ O Hollinger
Apr 1, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·D D HileM V Pishko
Nov 2, 2001·Nature Biotechnology·T P RichardsonD J Mooney
Dec 6, 2001·The Journal of Pharmacy and Pharmacology·M J WhitakerK M Shakesheff
Feb 8, 2005·Microsurgery·Brian C CooleyJeffrey M Toth
Apr 13, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yen-Chen HuangDavid J Mooney
Oct 22, 2005·Orthodontics & Craniofacial Research·O Arosarena, W Collins
Nov 22, 2005·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Philip J BoyneR Gilbert Triplett
Jun 30, 2006·Journal of Periodontology·Archie A JonesDavid L Cochran
Jan 18, 2007·Journal of Biomedical Materials Research. Part a·Po-Chun ChangLein-Tuan Hou
May 5, 2007·Journal of Biomedical Materials Research. Part a·H SchliephakeR Gruber

❮ Previous
Next ❯

Citations

Oct 30, 2009·Oral and Maxillofacial Surgery·Henning Schliephake
Oct 21, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·N LohseH Schliephake
May 15, 2015·Drug Delivery and Translational Research·Ethan NybergWarren L Grayson
Apr 1, 2018·Journal of Tissue Engineering and Regenerative Medicine·Maurits G L OlthofLichun Lu
Jul 10, 2010·Journal of Biomaterials Science. Polymer Edition·Juthamas RatanavarapornYasuhiko Tabata
Oct 26, 2018·Journal of Biomedical Materials Research. Part a·Andreas KolkChristian Plank
Jan 8, 2019·Tissue Engineering. Part a·Maurits G L OlthofLichun Lu
Apr 17, 2020·Journal of Biomedical Materials Research. Part a·Francesca VeronesiMilena Fini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.