Ectopic expression of Arabidopsis L-type lectin receptor kinase genes LecRK-I.9 and LecRK-IX.1 in Nicotiana benthamiana confers Phytophthora resistance

Plant Cell Reports
Yan WangKlaas Bouwmeester

Abstract

Transgenic Nicotiana benthamiana lines with constitutive expression of an Arabidopsis lectin receptor kinase gene (LecRK - I.9 or LecRK - IX.1) show enhanced resistance to Phytophthora pathogens, demonstrating conserved gene functionality after interfamily transfer. In plants, cell surface receptors mediate the first layer of innate immunity against pathogenic microbes. In Arabidopsis several L-type lectin receptor kinases (LecRKs) were previously found to function as Phytophthora resistance components. In this study, we determined the functionality of Arabidopsis LecRK-I.9 or LecRK-IX.1 in Phytophthora resistance when transferred into the Solanaceous plant Nicotiana benthamiana. Multiple transgenic lines were generated for each LecRK gene and molecular analyses revealed variation in transgene copy number, transgene expression levels and LecRK protein accumulation. Infection assays showed that transgenic N. benthamiana plants expressing either Arabidopsis LecRK-I.9 or LecRK-IX.1 are more resistant to Phytophthora capsici and to Phytophthora infestans. These results demonstrate that Arabidopsis LecRK-I.9 and LecRK-IX.1 retained their Phytophthora resistance function when transferred into N. benthamiana. Therefore, these LecRKs h...Continue Reading

References

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Sep 24, 2009·Journal of Experimental Botany·Klaas Bouwmeester, Francine Govers
Jan 1, 2007·Molecular Plant Pathology·Brett M Tyler
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Jun 21, 2014·Trends in Immunology·Cyril Zipfel

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Citations

Jun 1, 2017·International Journal of Molecular Sciences·Kevin BellandeHervé Canut
Feb 24, 2018·Biochemical Society Transactions·Sofie Van Holle, Els J M Van Damme
Aug 18, 2017·PLoS Pathogens·Yan Wang, Klaas Bouwmeester
Dec 14, 2019·Journal of Integrative Plant Biology·Xiaoqun PengXiaoyan Tang
Jun 6, 2019·BMC Genomics·Camila Campos MantelloAnete Pereira de Souza
Dec 29, 2020·Frontiers in Plant Science·Yali SunJin-Gui Chen
May 14, 2017·Current Opinion in Plant Biology·Stefanie Ranf
May 14, 2021·Nature Communications·Dongqin ChenGary Stacey

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Methods Mentioned

BETA
transgenic
PCR
phosphotransferase
immunoprecipitation
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